{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T23:01:31Z","timestamp":1785366091033,"version":"3.55.0"},"reference-count":54,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T00:00:00Z","timestamp":1600992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/24458\u20101"],"award-info":[{"award-number":["2015\/24458\u20101"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to determine the role of resveratrol (RESV) on the progression of experimental periodontitis (EP) in ovariectomy rats (OVT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Estrogen deficiency is the main cause of osteoporosis and is related to higher periodontal attachment loss and reduction of alveolar bone. Zoledronate (ZLD) is an antiresorptive drug used to control osteoporosis but can lead to osteonecrosis of the jaw. RESV, a natural product, can reduce bone loss and control and prevent osteoporosis. Thus, this study aimed at investigating the effect of RESV on the progression of EP in estrogen\u2010deficient rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>The animals were subjected to the OVT or sham surgery to induce estrogen\u2010deficiency and then were divided into the groups: OVT\u00a0+\u00a0RESV (n: 10); OVT\u00a0+\u00a0PLAC (n: 10): OVT\u00a0+\u00a0placebo; OVT\u00a0+\u00a0ZLD +PLA (n: 10); OVT\u00a0+\u00a0RESV +ZLD (n: 10): OVT\u00a0+\u00a0RESV and ZLD; SHAM (n: 10): non\u2010ovariectomized animals\u00a0+\u00a0placebo. To induce estrogen deficiency, the rats were subjected to ovariectomy. Experimental periodontitis was induced by the placement of a ligature at the second maxillary molars. Daily administration of the placebo solution, resveratrol (10\u00a0mg\/kg), and ZLD (0.1\u00a0mg\/kg) was carried out for a period 42\u00a0days prior to initiation of EP, and then for another 28\u00a0days following ligature placement. After euthanasia, the specimens were processed for micro\u2010CT and morphometric analysis of bone loss (linear measurement), and the gingival tissue surrounding the maxillary second molar was collected for the quantification of inflammatory markers using Luminex\/MAGPix, of oxidative stress markers using ELISA assay, and gene expression analysis of bone markers, by real\u2010time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Morphometric and micro\u2010CT analysis showed higher bone loss and lower bone density, respectively, in OVT\u00a0+\u00a0PLAC when compared to the other groups (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). ZLD treated groups had lower alveolar bone loss, as well as, higher density and percentage of bone volume, when compared to OVT\u00a0+\u00a0RESV and SHAM\u00a0+\u00a0PLAC groups (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). IL\u20104 levels were significantly lower in the OVT\u00a0+\u00a0PLAC group versus OVT\u00a0+\u00a0ZLD +RESV and SHAM\u00a0+\u00a0PLAC (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). NADPH oxidase (nicotinamide adenine dinucleotide phosphate oxidase) levels were significantly lower OVT\u00a0+\u00a0RESV group when compared to OVT\u00a0+\u00a0PLAC (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). OPG mRNA levels were lower in OVT\u00a0+\u00a0PLAC compared with the SHAM\u00a0+\u00a0PLAC group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>It can be concluded that resveratrol modulated alveolar bone loss during experimental periodontitis progression in estrogen\u2010deficient rats by downregulating NADPH oxidase levels.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12775","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T18:37:25Z","timestamp":1601059045000},"page":"840-849","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Effect of resveratrol on the progression of experimental periodontitis in an ovariectomized rat model of osteoporosis: Morphometric, immune\u2010enzymatic, and gene expression analysis"],"prefix":"10.1111","volume":"55","author":[{"given":"Andr\u00e9ia Manetta","family":"Molez","sequence":"first","affiliation":[{"name":"Dental Research Division School of Dentistry Paulista University S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduarda Helena Leandro","family":"do Nascimento","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis Division of Oral Radiology Piracicaba Dental School University of Campinas Piracicaba, S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Haiter Neto","sequence":"additional","affiliation":[{"name":"Department of Oral Diagnosis Division of Oral Radiology Piracicaba Dental School University of Campinas Piracicaba, S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7331-4612","authenticated-orcid":false,"given":"Fabiano Ribeiro","family":"Cirano","sequence":"additional","affiliation":[{"name":"Dental Research Division School 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