{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:04:41Z","timestamp":1791475481384,"version":"4.3.4"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,3,9]],"date-time":"2021-03-09T00:00:00Z","timestamp":1615248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Long non\u2010coding RNAs (lncRNAs) can act as competing endogenous RNAs (ceRNAs) to compete for micro\u2010RNAs (miRNAs) in regulation of downstream genes, various biological functions and diseases. Yet, the expression and regulation of lncRNAs in periodontitis are not fully understood. The objective of the study was to identify potential genes (lncRNA, messenger RNA [mRNA] and miRNA) involved in periodontitis, construct lncRNA\u2010miRNA\u2010mRNA ceRNA networks, explore gene functions and validate gene expressions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>The data sets for the lncRNA, mRNA and miRNA expression profiles in gingival samples from periodontally healthy subjects and chronic periodontitis patients were obtained from the Gene Expression Omnibus. The differentially expressed lncRNAs (DElncRNAs), mRNAs (DEmRNAs) and miRNAs (DEmiRNAs) were identified, and ceRNA networks were then constructed. The expression of DElncRNAs and DEmRNAs was examined by quantitative real\u2010time polymerase chain reaction (qPCR). Moreover, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed for exploring the potential functions and biological pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The GSE80715 and GSE54710 data sets were retrieved. Subsequently, 26 DElncRNAs, 436 DEmRNAs and 12 DEmiRNAs were identified (|fold change| \u22652, adjusted <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). Further bioinformatics analysis contributed to establishment of the ceRNA networks, which consisted of 10 DElncRNAs, 11 DEmiRNAs and 83 DEmRNAs. Notably, the qPCR results showed a marked decrease in the expression of lncRNA H19 and two mRNAs (NOS1 and MAPT) which further supported the identified ceRNA network. The GO results revealed that the up\u2010regulated mRNAs were significantly enriched in inflammatory processes, whilst the down\u2010regulated mRNAs were enriched in cellular potentials.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Non\u2010coding RNAs are critically involved in the regulatory mechanisms in the pathogenesis of periodontitis. Further study is warranted to investigate the specific underlying genetic traits and networks.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12864","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T07:00:54Z","timestamp":1615359654000},"page":"679-689","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Identification and integrated analysis of differentially expressed long non\u2010coding RNAs associated with periodontitis in humans"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2614-9159","authenticated-orcid":false,"given":"Yifan","family":"Lin","sequence":"first","affiliation":[{"name":"Orthodontics, Faculty of Dentistry the University of Hong Kong Hong Kong SAR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4305-1337","authenticated-orcid":false,"given":"Lijian","family":"Jin","sequence":"additional","affiliation":[{"name":"Periodontology &amp; Implant Dentistry, Faculty of Dentistry the University of Hong Kong Hong Kong SAR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wai Man","family":"Tong","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, Faculty of Dentistry the University of Hong Kong Hong Kong SAR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7038-3279","authenticated-orcid":false,"given":"Yiu Yan","family":"Leung","sequence":"additional","affiliation":[{"name":"Oral and Maxillofacial Surgery, Faculty of Dentistry the University of Hong Kong Hong Kong SAR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6447-9573","authenticated-orcid":false,"given":"Min","family":"Gu","sequence":"additional","affiliation":[{"name":"Orthodontics, Faculty of Dentistry the University of Hong Kong Hong Kong SAR"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3582-1277","authenticated-orcid":false,"given":"Yanqi","family":"Yang","sequence":"additional","affiliation":[{"name":"Orthodontics, Faculty of Dentistry the University of Hong Kong Hong Kong SAR"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67728-8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bj.2018.12.001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12428"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12732"},{"issue":"62","key":"e_1_2_8_6_1","first-page":"203","article-title":"Periodontitis: a host\u2010mediated disruption of microbial homeostasis. 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