{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T02:00:37Z","timestamp":1780970437355,"version":"3.54.1"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T00:00:00Z","timestamp":1616544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Root resorption is an unavoidable side effect of orthodontic tooth movement. The mechanism of root resorption is similar to bone resorption; the odontoclasts share similar characteristics with osteoclasts (OCs). MicroRNAs (miRNAs) such as miR\u2010155\u20105p play an important role in OC differentiation, but the underlying molecular mechanism of miR\u2010155\u20105p in this process is not fully understood. We found that the miR\u2010155\u20105p seed sequences were complementary to a sequence conserved in the 3\u2010untranslated region of CXCR2 mRNA. In this study, we explored the molecular mechanism underlying the effect of miR\u2010155\u20105p on OC differentiation by targeting CXCR2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>In this study, we divided the orthodontic patients into mild, moderate, and severe groups according to the severity of root resorption. The gingival crevicular fluid (GCF) of patients in different groups was collected, and the expression levels of dentin phosphoprotein (DPP) were detected by ELISA, and the expression levels of CXCR2 and miR\u2010155\u20105p in GCF were detected by real\u2010time quantitative PCR (qRT\u2010PCR). The relationship between miR\u2010155\u20105p and CXCR2 was verified by double luciferase. We analyzed changes of CXCR2 and miR\u2010155\u20105p expression after transfection of miR\u2010155\u20105p mimic and inhibitor into RAW264.7 cells induced by receptor activator of nuclear factor\u2010\u03baB ligand (RANKL) through qRT\u2010PCR and western blotting. The effect of miR\u2010155\u20105p on OC differentiation was evaluated by tartrate\u2010resistant acid phosphatase (TRAP) staining. QRT\u2010PCR and western blotting were used to analyze expression of the osteoclastic bone resorption\u2010related enzymes carbonic anhydrase 2 (CA II), matrix metalloproteinase\u20109 (MMP\u20109), and cathepsin K. To further confirm the direct targeting effect of CXCR2 by miR\u2010155\u20105p, we blocked CXCR2 using si\u2010CXCR2 in RANKL\u2010induced RAW264.7 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Dentin phosphoprotein levels were consistent with the trend of miR\u2010155\u20105p changes, and the trend of CXCR2 expression was opposite to miR\u2010155\u20105p changes. miR\u2010155\u20105p can be directly targeted to act on CXCR2. The expression of miR\u2010155\u20105p was significantly downregulated in differentiated OCs. MiR\u2010155\u20105p inhibited OC differentiation, and downregulated CA II, MMP\u20109, and cathepsin K expression at the protein and mRNA levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In summary, the results of this study suggested that miR\u2010155\u20105p inhibited OC differentiation by targeting CXCR2, thus reducing root resorption in orthodontics. MiR\u2010155\u20105p can be used as an effective target for avoiding or reducing the degree of root resorption in orthodontic treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12875","type":"journal-article","created":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T10:41:41Z","timestamp":1616582501000},"page":"761-773","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The miR\u2010155\u20105p inhibits osteoclast differentiation through targeting CXCR2 in orthodontic root resorption"],"prefix":"10.1111","volume":"56","author":[{"given":"Hongtao","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Orthodontics Dalian Stomatological Hospital Dalian China"},{"name":"Faculty of Orthodontics and Dentofacial Orthopedics Graduate School of Dentistry Tohoku University Sendai Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Kitaura","sequence":"additional","affiliation":[{"name":"Faculty of Orthodontics and Dentofacial Orthopedics Graduate School of Dentistry Tohoku University Sendai Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3023-3932","authenticated-orcid":false,"given":"Lin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Dalian Stomatological Hospital Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Itaru","family":"Mizoguchi","sequence":"additional","affiliation":[{"name":"Faculty of Orthodontics and Dentofacial Orthopedics Graduate School of Dentistry Tohoku University Sendai Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiying","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Dalian Stomatological Hospital Dalian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-9657.2003.00192.x"},{"issue":"1","key":"e_1_2_9_3_1","first-page":"22","article-title":"Types of root resorptions related to orthodontic treatment","volume":"21","author":"Ru\u0161kyt\u0117 G","year":"2019","journal-title":"Stomatologija"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-018-0579-2"},{"issue":"2","key":"e_1_2_9_5_1","first-page":"175","article-title":"Orthodontically induced inflammatory root resorption. 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