{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T06:22:38Z","timestamp":1772518958240,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,13]],"date-time":"2021-11-13T00:00:00Z","timestamp":1636761600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670976"],"award-info":[{"award-number":["81670976"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Periodontitis is a chronic inflammatory disease of periodontal supporting tissues. The persistent inflammatory reaction depends on the release of chemokines to continuously recruit inflammation cells. GATA\u2010binding protein 4 (GATA4) exerts effects on senescence and inflammation, while its role in periodontitis is far from clear. The present study aims to address the effect of GATA4 on regulating chemokines and the chemotaxis in periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Periodontitis rat models were constructed to detect the expression of GATA4 and the chemokine monocyte chemoattractant protein\u20101 (MCP\u20101) by immunohistochemistry. Lipopolysaccharide (LPS)\u2010stimulated human periodontal ligament (PDL) cells and GATA4\u2010knockdown by siRNA transient transfection PDL cells were used to explore the correlation between GATA4 and chemokines. Transwell assay was performed to detect the role of GATA4 for the recruitment effect of chemokines on macrophages. Mitogen\u2010activated protein kinase (MAPK) inhibitors were scheduled to intervene in LPS\u2010stimulated PDL cells to examine the association between MAPK signaling pathways and GATA4. The expression of GATA4, chemokines, or MAPK signaling molecules was determined by quantitative real\u2010time polymerase chain reaction, western blotting, or cell immunofluorescence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of GATA4 and MCP\u20101 was significantly increased in periodontitis rat models and in LPS\u2010stimulated PDL cells. Knockdown GATA4 inhibited the expression of GATA4 and MCP\u20101 as well as suppressed the recruitment of macrophage in LPS\u2010stimulated PDL cells. Inhibitors of p38 and ERK1\/2 signaling pathways significantly downregulated the increased expression of GATA4 and MCP\u20101 induced by LPS in PDL cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>GATA\u2010binding protein 4 could act as an upstream regulator of MCP\u20101 and as a downstream regulator of p38 and ERK1\/2 signaling pathways to initiate inflammation response and regulate chemotaxis during the progression of periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12953","type":"journal-article","created":{"date-parts":[[2021,11,13]],"date-time":"2021-11-13T15:50:37Z","timestamp":1636818637000},"page":"195-204","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Activation of GATA\u2010binding protein 4 regulates monocyte chemoattractant protein\u20101 and chemotaxis in periodontal ligament cells"],"prefix":"10.1111","volume":"57","author":[{"given":"Ningjing","family":"Zhu","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei_MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueqing","family":"Zheng","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei_MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiwei","family":"Qiao","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei_MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wushuang","family":"Huang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei_MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiqi","family":"Li","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei_MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0747-7381","authenticated-orcid":false,"given":"Yaling","family":"Song","sequence":"additional","affiliation":[{"name":"The State Key Laboratory 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