{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T02:47:24Z","timestamp":1791859644313,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T00:00:00Z","timestamp":1644364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100014103","name":"Key Research and Development Program of Shandong Province","doi-asserted-by":"publisher","award":["2019GSF107001"],"award-info":[{"award-number":["2019GSF107001"]}],"id":[{"id":"10.13039\/100014103","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Occlusal trauma is considered to be a contributing factor to bone loss associated with inflammatory periodontal disease. We hypothesized that pyroptosis, a recently discovered inflammation\u2010induced programmed cell death pathway, plays a role in occlusal trauma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The occlusal trauma model was established using a cemented 1\u2010mm elevated computer\u2010aided design and manufacturing (CAD\/CAM) metal crown. The periodontitis model was established by periodontal wire ligation with lipopolysaccharide (LPS) injection. The rats were sacrificed at 1, 2, 3, and 4\u00a0weeks. Quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) was used to analyze the expression of pyroptosis\u2010, inflammation\u2010, and osteoclast\u2010related markers. Micro\u2010computed tomography (micro\u2010CT) was used to determine bone morphology parameters. Tissue morphology was evaluated using hematoxylin and eosin staining (H&amp;E). Osteoclasts were identified using tartrate\u2010resistant acid phosphatase (TRAP) staining. The expression and distribution of factors related to pyroptosis and inflammation were evaluated by immunohistochemistry (IHC). The colocalization of dead cells and cysteinyl aspartate\u2013specific proteinase\u20101 (caspase\u20101)\u2010positive cells was analyzed by immunofluorescence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Quantitative real\u2010time polymerase chain reaction and IHC results showed that occlusal trauma induced the expression of pyroptotic factors during the early stages, while occlusal trauma with periodontitis upregulated the expression of pyroptotic factors at the later stages. The results of qRT\u2010PCR, TRAP staining, and micro\u2010CT showed that occlusal trauma with periodontitis increased the production of proinflammatory cytokines, leading to severe bone loss. Glyburide, an NOD\u2010like receptor pyrin domain containing protein 3 (NLRP3)inhibitor, reduced the expression of pyroptosis markers induced by occlusal trauma with periodontitis and reversed bone resorption.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Pyroptosis was involved in bone loss induced by occlusal trauma with or without periodontitis, while glyburide reversed inflammation and bone resorption.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12974","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T05:40:15Z","timestamp":1644471615000},"page":"448-460","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Study on the role of pyroptosis in bone resorption induced by occlusal trauma with or without periodontitis"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8709-8797","authenticated-orcid":false,"given":"Mengyang","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Prosthodontics School and Hospital of Stomatology Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration  Jinan China"},{"name":"Department of Stomatology Songjiang Sijing Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics School and Hospital of Stomatology Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration  Jinan China"},{"name":"Department of Stomatology Songjiang Sijing Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics School and Hospital of Stomatology Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojie","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics School and Hospital of Stomatology Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Liang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases National Clinical Research Center for Oral Diseases West China Hospital of Stomatology Sichuan University  Chengdu China"},{"name":"Department of Prosthodontics West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8878-4676","authenticated-orcid":false,"given":"Huiqiang","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics School and Hospital of Stomatology Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,9]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"1330","article-title":"What is traumatic occlusion and how can it be diagnosed and corrected?","volume":"12","author":"Stillman PR","year":"1922","journal-title":"J Am Dent Assoc"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jopr.13235"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.16-0581"},{"key":"e_1_2_11_5_1","unstructured":"KarolyiM.Beobachtungen \u00fcber pyorrhea alveolaris(Observation of chronic periodontitis). \u00d6st. 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