{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T11:05:37Z","timestamp":1788260737302,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T00:00:00Z","timestamp":1650844800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Identification of biomarkers to assess individual risk and monitor periodontal health status is important. Research on lipocalin\u20102 (LCN2) and semaphorin3A (Sema3A) is lacking. This study aimed to evaluate gingival crevicular fluid (GCF) LCN2, Sema3A, and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) levels in periodontally healthy (H), gingivitis (G), and periodontitis (P) patients, and their changes following non\u2010surgical periodontal therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty systemically healthy and non\u2010smoker participants, diagnosed as periodontally healthy, gingivitis, and stage III grade C periodontitis, were recruited (<jats:italic>n<\/jats:italic>\u00a0=\u00a020\/group). Clinical periodontal parameters were recorded and GCF samples were obtained at baseline from all groups; for group P, these were repeated one and three months following non\u2010surgical periodontal treatment. GCF LCN2, Sema3A, and TNF\u2010\u03b1 levels were evaluated with enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>GCF LCN2, Sema3A, and TNF\u2010\u03b1 total amounts were significantly higher in disease groups than group H (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001). Between P and G groups, only TNF\u2010\u03b1 levels were significantly different (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001). Non\u2010surgical periodontal therapy resulted in significant improvement of all clinical parameters and significant decreases of GCF LCN2 and TNF\u2010\u03b1 levels, at both time points, compared with baseline (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001). Sema3A levels remained unchanged following treatment (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). LCN2 and TNF\u2010\u03b1 levels were significantly positively correlated with clinical parameters. LCN2 (AUC [area under the curve]\u00a0=\u00a00.94) and TNF\u2010\u03b1 (AUC\u00a0=\u00a00.98) levels were similarly accurate in differentiating between periodontal disease (whether G or P) and healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>LCN2 and TNF\u2010\u03b1 levels in GCF are correlated with clinical parameters and could prove useful as non\u2010invasive screening tools for periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.12995","type":"journal-article","created":{"date-parts":[[2022,4,25]],"date-time":"2022-04-25T17:54:53Z","timestamp":1650909293000},"page":"724-732","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Gingival crevicular fluid lipocalin\u20102 and semaphorin3A in stage III periodontitis: Non\u2010surgical periodontal treatment effects"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3947-5519","authenticated-orcid":false,"given":"Merve","family":"Ceylan","sequence":"first","affiliation":[{"name":"Department of Periodontology Faculty of Dentistry Izmir Katip Celebi University  Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8995-5561","authenticated-orcid":false,"given":"Huriye","family":"Erbak Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry School of Medicine Izmir Katip Celebi University  Izmir Turkey"},{"name":"Izmir Biomedicine Genome Center Dokuz Eylul University  Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2643-9575","authenticated-orcid":false,"given":"Figen","family":"Narin","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry School of Medicine Izmir Katip Celebi University  Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6327-3610","authenticated-orcid":false,"given":"Dimitris N.","family":"Tatakis","sequence":"additional","affiliation":[{"name":"Division of Periodontology College of Dentistry The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2703-2462","authenticated-orcid":false,"given":"Mehmet","family":"Saglam","sequence":"additional","affiliation":[{"name":"Department of Periodontology Faculty of Dentistry Izmir Katip Celebi University  Izmir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3785"},{"issue":"25","key":"e_1_2_10_3_1","first-page":"8","article-title":"Causation and pathogenesis of periodontal 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