{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T21:46:57Z","timestamp":1792705617845,"version":"4.5.0"},"reference-count":57,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700982"],"award-info":[{"award-number":["81700982"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Published studies proved that both pyroptosis and periodontitis owned a substantial relationship with immunity, and recent research revealed a solid correlation between periodontitis and pyroptosis. While abundant findings have confirmed pyroptosis has a strong impact on the tumor microenvironment, the function of pyroptosis in influencing the periodontitis immune microenvironment remains poorly understood. Thus, we aimed to identify pyroptosis\u2010related genes whose expression signature can well discriminate periodontitis from healthy controls and to comprehend the role of pyroptosis in the periodontitis immune microenvironment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>The periodontitis\u2010related datasets were acquired from the Gene Expression Omnibus (GEO) database. A series of bioinformatics analyses were conducted to investigate the underlying mechanism of pyroptosis in the periodontitis immune microenvironment. Infiltrating immunocytes, immunological reaction gene sets, and the human leukocyte antigen (HLA) gene were all investigated as potential linkages between periodontitis immune microenvironment and pyroptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010one pyroptosis\u2010related genes were dysregulated. A four\u2010mRNA combined classification model was constructed, and the receiver operating characteristic (ROC) curve analysis demonstrated its prominent classification capabilities. Subsequently, the mRNA levels of the four hub markers (CYCS, CASP3, NOD2, CHMP4B) were validated by quantitative real\u2010time PCR (qRT\u2010PCR). The correlation coefficients between each hub gene and immune characteristics were calculated, and CASP3 exhibited the most significant correlations with the immune characteristics. Furthermore, distinct pyroptosis\u2010related expression patterns were revealed, along with immunological features of each pattern. Afterward, we discovered 1868 pyroptosis phenotype\u2010related genes, 134 of which were related to immunity. According to the functional enrichment analysis, these 134 genes were closely related to cytokine signaling in immune system, and defense response. Finally, a co\u2010expression network was constructed via the 1868 gene expression profiles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Four hub mRNAs (CYCS, CASP3, NOD2, and CHMP4B) formed a classification model and concomitant results revealed the crucial role of pyroptosis in the periodontitis immune microenvironment, providing fresh insights into the etiopathogenesis of periodontitis and potential immunotherapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13035","type":"journal-article","created":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T17:38:37Z","timestamp":1657906717000},"page":"977-990","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Pyroptosis may play a crucial role in modifications of the immune microenvironment in periodontitis"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6835-5735","authenticated-orcid":false,"given":"Hang","family":"Chen","sequence":"first","affiliation":[{"name":"College of Stomatology Chongqing Medical University  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2518-1528","authenticated-orcid":false,"given":"Zhenxiang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Stomatology Chongqing Medical University  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3349-8082","authenticated-orcid":false,"given":"Yujuan","family":"He","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Key Laboratory of Diagnostic Medicine, (Ministry of Education) Chongqing Medical University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0162-438X","authenticated-orcid":false,"given":"Limin","family":"Peng","sequence":"additional","affiliation":[{"name":"College of Stomatology Chongqing Medical University  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junlin","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Stomatology Chongqing Medical University  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0155-776X","authenticated-orcid":false,"given":"Xiaonan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Stomatology Chongqing Medical University  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67728-8"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2337"},{"issue":"14","key":"e_1_2_10_4_1","first-page":"S106","article-title":"Working group 2 of the joint EFPAAPw. 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