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Scaffolds were characterized for their morphology and biological performance by scanning electron microscopy (SEM), confocal and conventional histology, cytocompatibility (PrestoBlue assay), gene expression (type I collagen \u2013 <jats:italic>Col1<\/jats:italic>; bone sialoprotein \u2013 <jats:italic>Bsp<\/jats:italic>; runt\u2010related transcription factor 2 \u2013 <jats:italic>Runx\u20102<\/jats:italic>; alkaline phosphatase \u2013 <jats:italic>Alpl<\/jats:italic>; osteopontin \u2013 <jats:italic>Opn<\/jats:italic>; osteocalcin \u2013 <jats:italic>Ocn<\/jats:italic>, osterix \u2013 <jats:italic>Osx<\/jats:italic>), and the potential to induce extracellular matrix deposition and mineralization in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, data analysis showed that PCL scaffolds allowed cell adhesion and proliferation, modulated the expression of key markers of cementoblasts, and led to enhanced extracellular matrix deposition and calcium deposition as compared to the control group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Altogether, our findings allow concluding that PCL scaffolds are a viable tool to culture OCCM\u201030 cells, leading to an increased potential to promote mineralization in vitro. Further studies should be designed in order to define the clinical relevance of cementoblast\u2010loaded PCL scaffolds to promote new cementum formation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13041","type":"journal-article","created":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T18:43:35Z","timestamp":1659725015000},"page":"1014-1023","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Polycaprolactone scaffolds as a biomaterial for cementoblast delivery: An in vitro study"],"prefix":"10.1111","volume":"57","author":[{"given":"Henrique B.","family":"Abdalla","sequence":"first","affiliation":[{"name":"S\u00e3o Leopoldo Mandic Medical and Dental School  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rayssa R.","family":"Marchioro","sequence":"additional","affiliation":[{"name":"S\u00e3o Leopoldo Mandic Medical and Dental School  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen Elizabeth A.","family":"Galv\u00e3o","sequence":"additional","affiliation":[{"name":"3D Biotechnology Solutions  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas\u00a0N.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"S\u00e3o Leopoldo Mandic Medical and Dental School  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamila R.","family":"Kantovitz","sequence":"additional","affiliation":[{"name":"S\u00e3o Leopoldo Mandic Medical and Dental School  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Luiza G. 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