{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:30:37Z","timestamp":1787761837084,"version":"build-2784847793"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T00:00:00Z","timestamp":1660003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771105"],"award-info":[{"award-number":["81771105"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare miRNA expression levels and predict relevant target genes and signaling pathways in peri\u2010implantitis and periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>There are many differences between periodontitis and peri\u2010implantitis. An understanding of the similarities and differences in the transcriptional patterns of these diseases, as well as the molecular mechanisms, is beneficial for the development of management strategies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Rat models of periodontitis (PD, <jats:italic>n<\/jats:italic>\u00a0=\u20096) and peri\u2010implantitis (PI, <jats:italic>n<\/jats:italic>\u00a0=\u20095) were established by ligation. Implantation without ligation (PIC, <jats:italic>n<\/jats:italic>\u00a0=\u20095) and normal rats (PDC, <jats:italic>n<\/jats:italic>\u00a0=\u20096) were used as controls. Micro\u2010CT was used to confirm the successful establishment of the model. Gingiva was harvested for miRNA transcriptome sequencing, and the results were confirmed by qRT\u2013PCR. miRNA target genes were predicted with miRTarBase. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sixty\u2010nine miRNAs were differentially expressed in PI vs. PD, 105 were differentially expressed in PI vs. PIC, and 70 were differentially expressed in PD vs. PDC (log<jats:sub>2<\/jats:sub>FC\u2009\u22651 and padj &lt;0.05). The upregulated genes in all three comparisons were mostly involved in the biological process response to stimulus, whereas most of the downregulated genes were involved in nervous system development (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.01). The upregulated genes in PI vs. PD and PI vs. PIC were involved in Toll\u2010like receptor signaling and RIG\u2010I\u2010like signaling. The upregulated genes in PI vs. PD were involved in T\u2010 and B\u2010cell receptor signaling, apoptosis, and osteoclast differentiation. Focal adhesion was downregulated in all three comparisons, and adherens junction was downregulated in PI vs. PD and PD vs. PDC (<jats:italic>p<\/jats:italic>\u00a0&lt;\u2009.1).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study showed differences in the miRNA expression profiles between peri\u2010implantitis and periodontitis and annotated the possible target genes and molecular mechanisms; this study could lay a foundation for the development of management strategies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13045","type":"journal-article","created":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T18:40:05Z","timestamp":1660070405000},"page":"1043-1055","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["<scp>MicroRNA<\/scp> sequence and function analysis in peri\u2010implantitis and periodontitis: An animal 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