{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T08:54:43Z","timestamp":1767084883367,"version":"3.40.5"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T00:00:00Z","timestamp":1661126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009592","name":"Beijing Municipal Science and Technology Commission","doi-asserted-by":"publisher","award":["Z16100000516203"],"award-info":[{"award-number":["Z16100000516203"]}],"id":[{"id":"10.13039\/501100009592","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81600891"],"award-info":[{"award-number":["81600891"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>The potential role of the transcription factor Differentiated embryo\u2010chondrocyte 2 (Dec2) in the progression of inflammatory diseases such as periodontitis has been unclear. Here, the effect of Dec2 on the expression of RANKL and on osteoclastogenesis was determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>Wild\u2010type (WT) and <jats:italic>Dec2<\/jats:italic> knockout (KO) mice as a model for periodontitis were used to assess alveolar bone resorption by microcomputed tomography (CT). Western blot, flow cytometry, quantitative real\u2010time PCR, and immunohistochemical analyses were utilized to detect inflammation and osteoclasts. Luciferase reporter and Chromatin immunoprecipitation (ChIP) assays examined the interaction between Dec2 and RANKL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Micro\u2010CT showed that the alveolar bone resorption of <jats:italic>Dec2<\/jats:italic>KO mice was more severe than WT mice after treatment with <jats:italic>P.\u00a0gingivalis<\/jats:italic>. Immunohistochemistry and Tartrate\u2010resistant acid phosphatase staining showed active osteoclast differentiation in <jats:italic>Dec2<\/jats:italic>KO mice. There was an increase in CD11b<jats:sup>+<\/jats:sup>F4\/80<jats:sup>+<\/jats:sup> and CD4<jats:sup>+<\/jats:sup>RANKL<jats:sup>+<\/jats:sup> T cells in <jats:italic>Dec2<\/jats:italic>KO mice treated with <jats:italic>P.\u00a0gingivalis<\/jats:italic>. Moreover, inflammatory and immune markers were expressed at significantly higher levels in gingival mononuclear cells in <jats:italic>Dec2<\/jats:italic>KO mice. Furthermore, luciferase reporter and ChIP assays confirmed the direct binding of Dec2 protein to the RANKL gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Dec2 has an immune regulation ability that modulates <jats:italic>P.\u00a0gingivalis<\/jats:italic>\u2010induced periodontitis via RANKL.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13046","type":"journal-article","created":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T07:16:56Z","timestamp":1661152616000},"page":"1056-1069","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dec2 negatively regulates bone resorption in periodontitis"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7166-0135","authenticated-orcid":false,"given":"Xiaoyan","family":"Li","sequence":"first","affiliation":[{"name":"Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijia","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, School of Stomatology Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuyuki","family":"Sato","sequence":"additional","affiliation":[{"name":"Pathology Division Shizuoka Cancer Center  Shizuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiyasu","family":"Kitayama","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Nihon University School of Dentistry  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nitesh","family":"Tewari","sequence":"additional","affiliation":[{"name":"Division of Pedodontics and Preventive Dentistry Centre for Dental Education and Research, All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makoto","family":"Makishima","sequence":"additional","affiliation":[{"name":"Division of Biochemistry, Department of Biomedical Sciences Nihon University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobushiro","family":"Hamada","sequence":"additional","affiliation":[{"name":"Department of Oral Microbiology Kanagawa Dental University  Yokosuka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4998-5547","authenticated-orcid":false,"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology Capital Medical University  Beijing China"},{"name":"Immunology Research Center for Oral and Systematic Health, Beijing Friendship Hospital Capital Medical University  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3746-009X","authenticated-orcid":false,"given":"Ujjal K.","family":"Bhawal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Nihon University School of Dentistry at Matsudo  Chiba Japan"},{"name":"Department of Pharmacology, Saveetha Dental College Saveetha Institute of Medical and Technical Sciences  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,22]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"925","article-title":"Transcriptional repression by the basic\u2010helix\u2010loop\u2010helix protein Dec2: multiple mechanisms through E\u2010box elements","volume":"19","author":"Fujimoto K","year":"2007","journal-title":"Int J Mol 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