{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T23:07:22Z","timestamp":1778022442178,"version":"3.51.4"},"reference-count":76,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T00:00:00Z","timestamp":1663804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100018525","name":"Key Research and Development Program of Sichuan Province","doi-asserted-by":"publisher","award":["20ZDYF3012"],"award-info":[{"award-number":["20ZDYF3012"]}],"id":[{"id":"10.13039\/501100018525","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970948"],"award-info":[{"award-number":["81970948"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Pyroptosis has both a caspase\u20101\u2010dependent canonical pathway and a caspase\u20104\/\u20105\/\u201011\u2010dependent noncanonical pathway. They play an important role in inflammatory damage and related diseases. Canonical pyroptosis was reported to be involved in periodontitis. However, knowledge of caspase\u20104\/\u20105\/\u201011\u2010dependent noncanonical pathway involvement remains limited. The aim of this study was to investigate the outcomes of pyroptosis inhibition on periodontitis as well as the possible mechanism, in order to provide a potential target for alleviating periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human and rat periodontitis tissues were collected for immunohistochemistry (IHC). Micro\u2010computed tomography was used to assess alveolar bone loss in experimental periodontitis model. Pyroptosis\u2010related proteins were tested by western blot. propidium iodide staining and lactate dehydrogenase release were used to verify pyroptosis activation. RNA sequencing was applied to investigate the preliminary mechanism of the reduced periodontal inflammation induced by YVAD\u2010CHO.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both canonical\u2010 and noncanonical\u2010related proteins were detected in human and rat periodontitis tissue. The pyroptosis\u2010inhibited group demonstrated less inflammatory response and bone absorption. In vitro, pyroptosis was activated by lipopolysaccharide and inhibited by YVAD\u2010CHO. RNA sequencing demonstrated that the expression of A20 and I\u03baB\u2010\u03b6 was increased and verified by western blot in vitro and IHC in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that inhibition of pyroptosis\u2010reduced inflammation and alveolar bone resorption in periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13055","type":"journal-article","created":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T05:18:40Z","timestamp":1663910320000},"page":"1183-1197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Canonical and noncanonical pyroptosis are both activated in periodontal inflammation and bone resorption"],"prefix":"10.1111","volume":"57","author":[{"given":"Rui","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases and Department of 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