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And recently, periodontal ligament\u2010associated protein\u20101 (PLAP\u20101)\/asporin was found to play a pivotal role in maintaining periodontal homeostasis. The aim of this study was to explore the function of PLAP\u20101 in the periodontally hypofunctional tissue turnover.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>After extracting left maxillary molars in mice, the left and right mandibular molars were distributed into hypofunction group (HG) and control group (CG), respectively. Mice were sacrificed for radiographic, histological, and molecular biological analyses after 1, 4 and 12\u2009weeks. In vitro, dynamic compression was applied using Flexcell FX\u20105000 Compression System to simulate intermittent occlusal force. The expression of <jats:italic>PLAP1<\/jats:italic> in loaded and unloaded human periodontal ligament cells (hPDLCs) was compared, and its molecular biological effects were further explored by small interfering RNA (siRNA) targeting <jats:italic>PLAP1<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In vivo, fiber disorder in periodontal ligament (PDL), bone apposition at furcation regions, and bone resorption in alveolar bone were illustrated in the HG compared with the CG. In addition, PLAP\u20101 positive area decreased significantly in PDL following occlusal unloading. In vitro, the loss of compressive loading relatively downregulated <jats:italic>PLAP1<\/jats:italic> expression, which was essential to promote collagen I but inhibit osterix and osteocalcin expression in hPDLCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PLAP\u20101 presumably plays a pivotal role in occlusal force\u2010regulated periodontal homeostasis by facilitating collagen fiber synthesis in hPDLCs and suppressing excessive osteoblast differentiation, further preventing teeth from overeruption. Further evidence in PLAP\u20101 conditional knockout mice is needed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13075","type":"journal-article","created":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T19:15:40Z","timestamp":1669749340000},"page":"131-142","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Periodontal ligament\u2010associated protein\u20101 engages in teeth overeruption and periodontal fiber disorder following occlusal hypofunction"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0054-9143","authenticated-orcid":false,"given":"Yilin","family":"Chen","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University  Chengdu China"},{"name":"Department of Orthodontics and Pediatric Dentistry, West China Hospital of Stomatology Sichuan 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