{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T12:30:31Z","timestamp":1781353831683,"version":"3.54.1"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,1,8]],"date-time":"2023-01-08T00:00:00Z","timestamp":1673136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background &amp; Objective<\/jats:title><jats:p>Notch signaling pathway has been linked to bone loss in periodontitis and peri\u2010implantitis. This research aimed to determine the Notch signaling molecules expression levels (Notch1, Notch2, Jagged1, Hes1, and Hey1), along with bone remodeling mediators (RANKL and OPG) and proinflammatory cytokines (TNF\u2010\u03b1, IL\u201017, IL\u20101\u03b2, and IL\u20106) in patients with peri\u2010implant diseases. The aforementioned markers' expression was evaluated in patients with different RANKL\/OPG ratios.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fifty patients with peri\u2010implantitis (PI group) and 45 patients with peri\u2010implant mucositis (PM group) were enrolled. Relative gene expression levels of investigated molecules were determined by reverse transcriptase\u2010real\u2010time polymerase chain reaction. On the basis of RANKL\/OPG ratio, all peri\u2010implant lesions were divided into subgroups: RANKL\u2010predominant (RANKL\u2009&gt;\u2009OPG) and OPG\u2010predominant (RANKL\u2009&lt;\u2009OPG). Clinical periodontal parameters (probing depth\u2010PD, bleeding on probing\u2010BOP, clinical attachment level\u2010CAL and plaque index\u2010PLI), were recorded for each patient around every tooth, and around placed implants (PDi, BOPi, CALi, PLIi).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RANKL\u2010predominant PM patients exhibited higher expression levels of Notch2 (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.044) and Hey1 (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.005) compared to OPG\u2010predominant lesions. In all RANKL\u2010predominant cases, Hey1 (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.001), IL\u20101\u03b2 (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.005), IL\u20106 (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.002) were overexpressed in PI comparing to PM, accompanied with significantly higher PDi, CALi and PLIi in PI than PM (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.001, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.001 and <jats:italic>p<\/jats:italic>\u00a0=\u00a0.009).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Notch2 upregulation in RANKL\u2010predominant PM lesions could be an important contributor to alveolar bone resorption and represent a predictor of PM to PI transition. Similarly, the overexpression of IL\u20101\u03b2 and IL\u20106 might provide an osteoclastogenic environment in PI RANKL\u2010predominant lesions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13096","type":"journal-article","created":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T03:34:15Z","timestamp":1673235255000},"page":"360-368","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Notch signalling cascade and proinflammatory mediators in peri\u2010implant lesions with different RANKL\/OPG ratios\u2014An observational study"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9439-0529","authenticated-orcid":false,"given":"Ana","family":"Djinic\u00a0Krasavcevic","sequence":"first","affiliation":[{"name":"Department of Periodontology and Oral Medicine, School of Dental Medicine University of Belgrade  Belgrade 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