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HPDLCs are primary cells that can repair periodontal tissue defects caused by periodontitis. However, the inflammatory conditions induce inflammatory damage and decrease ossification of hPDLCs. This inflammatory response depends on genetic and epigenetic mechanisms, including m6A methylation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HPDLCs were cultured with osteogenic induction medium (NC group), while TNF\u2010\u03b1 (10\u00a0ng\/mL) and IL\u20101\u03b2 (5\u00a0ng\/mL) were added to simulate inflammatory conditions (Inflam group). Then RNA\u2010seq and MeRIP\u2010seq analyses were performed to identify m6A methylation modification in the transcriptome range of hPDLCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results showed that the osteogenic differentiation of hPDLCs was inhibited under inflammatory conditions. RNA\u2010seq analysis also revealed that the decreased genes in response to inflammatory conditions were primarily annotated in processes associated with ossification. Compared with the NC group, differentially m6A\u2010methylated genes were primarily enriched in histone modification processes. Among 145 histone modification genes, 25 genes have been reported to be involved in the regulation of osteogenic differentiation, and they include KAT6B, EP300, BMI1, and KDMs (KDM1A, KDM2A, KDM3A, KDM4B, and KDM5A).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrated that the m6A landscape of hPDLCs was changed in response to inflammation. M6A methylation differences among histone modification genes may act on the osteogenic differentiation of hPDLCs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13105","type":"journal-article","created":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T06:15:08Z","timestamp":1675404908000},"page":"444-455","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Changes in <scp>N6<\/scp>\u2010methyladenosine <scp>RNA<\/scp> methylomes of human periodontal ligament cells in response to inflammatory conditions"],"prefix":"10.1111","volume":"58","author":[{"given":"Xihong","family":"Zou","sequence":"first","affiliation":[{"name":"Department of Periodontology Nanjing Stomatological Hospital, Medical School of Nanjing University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoyi","family":"Liu","sequence":"additional","affiliation":[{"name":"Hangzhou Stomatological Hospital  Hangzhou 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