{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:09:52Z","timestamp":1792789792795,"version":"4.5.0"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T00:00:00Z","timestamp":1681257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2017YFA0701302"],"award-info":[{"award-number":["2017YFA0701302"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>The keratinized gingiva plays an important role in maintaining healthy periodontal and peri\u2010implant tissue. Acellular dermal matrix (ADM), as a substitute biomaterial, has a porous structure and good biocompatibility. 3D\u2010bioprinting has the potential for tissue engineering because it enables precise loading of cells layer\u2010by\u2010layer. Herein, we bioprinted ADM scaffold encapsulating gingival fibroblasts (GFs) and evaluated its efficacy in keratinized gingiva augmentation in vivo to assess its potential for clinical periodontal tissue regeneration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>GFs were extracted from the gingiva of beagles and transfected with a green fluorescent protein (GFP). The ADM scaffold (ADM cell\u2010free group) was constructed using ADM, gelatin, and sodium alginate mixed at an appropriate ratio via 3D\u2010bioprinting. The ADM cell scaffold (ADM cell group) was established by adding extra GFs in the same manner. Six beagles were divided into blank control, ADM cell\u2010free, and ADM cell groups; and implant surgery was performed. The keratinized gingiva was clinically and histologically evaluated at baseline and after 2\u2009months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>GFs transfected with GFPs expressed green fluorescence and were present in new tissue in the ADM cell group and not observed in the ADM cell\u2010free group. At 2\u2009months after surgery, the keratinized gingival augmentation in the ADM cell group was significantly more than that in the ADM cell\u2010free group. Attached gingival augmentation was also observed more in the ADM cell group than that in the ADM cell\u2010free group. Histological staining showed that the tissue in the ADM cell group displayed a more integrated structure and higher expression of COL I, COL III, and VEGF\u2010A than those in the ADM cell\u2010free group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>3D\u2010bioprinted GF\u2010encapsulated ADM scaffolds increased the amount of keratinized gingiva in vivo, suggesting that 3D\u2010bioprinting has great potential for oral soft tissue regeneration.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13126","type":"journal-article","created":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T07:30:31Z","timestamp":1681284631000},"page":"564-574","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Evaluation of the effect of<scp>3D<\/scp>\u2010bioprinted gingival fibroblast\u2010encapsulated<scp>ADM<\/scp>scaffolds on keratinized gingival augmentation"],"prefix":"10.1111","volume":"58","author":[{"given":"Peng","family":"Liu","sequence":"first","affiliation":[{"name":"Second Clinical Division Peking University School and Hospital of Stomatology Beijing 100101 P. 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