{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T21:28:27Z","timestamp":1771709307166,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T00:00:00Z","timestamp":1683504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Severe periodontitis causes alveolar bone resorption, resulting in tooth loss. Developments of tissue regeneration therapy that can restore alveolar bone mass are desired for periodontal disease. The application of bone morphogenetic protein\u20102 (BMP\u20102) has been attempted for bone fractures and severe alveolar bone loss. BMP\u20102 reportedly induces sclerostin expression, an inhibitor of Wnt signals, that attenuates bone acquisition. However, the effect of sclerostin\u2010deficiency on BMP\u20102\u2010induced bone regeneration has not been fully elucidated. We investigated BMP\u20102\u2010induced ectopic bones in <jats:italic>Sost<\/jats:italic>\u2010knockout (KO) mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>rhBMP\u20102 were implanted into the thighs of C57BL\/6 (WT) and <jats:italic>Sost<\/jats:italic>\u2010KO male mice at 8\u2009weeks of age. The BMP\u20102\u2010induced ectopic bones in these mice were examined on days 14 and 28 after implantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunohistochemical and quantitative RT\u2010PCR analyses showed that BMP\u20102\u2010induced ectopic bones expressed sclerostin in osteocytes on days 14 and 28 after implantation in <jats:italic>Sost\u2010Green<\/jats:italic> reporter mice. Micro\u2010computed tomography analysis revealed that BMP\u20102\u2010induced ectopic bones in <jats:italic>Sost<\/jats:italic>\u2010KO mice showed a significant increased relative bone volume and bone mineral density (WT\u2009=\u2009468\u2009mg\/cm<jats:sup>3<\/jats:sup>, <jats:italic>Sost<\/jats:italic>\u2010KO\u2009=\u2009602\u2009mg\/cm<jats:sup>3<\/jats:sup>) compared with those in WT mice on day 14 after implantation. BMP\u20102\u2010induced ectopic bones in <jats:italic>Sost<\/jats:italic>\u2010KO mice showed an increased horizontal cross\u2010sectional bone area on day 28 after implantation. Immunohistochemical staining showed that BMP\u20102\u2010induced ectopic bones in <jats:italic>Sost<\/jats:italic>\u2010KO mice had an increased number of osteoblasts with osterix\u2010positive nuclei compared with those in WT mice on days 14 and 28 after implantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Sclerostin deficiency increased bone mineral density in BMP\u20102\u2010induced ectopic bones.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13134","type":"journal-article","created":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T11:45:11Z","timestamp":1683546311000},"page":"769-779","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Sclerostin deficiency effectively promotes bone morphogenetic protein\u20102\u2010induced ectopic bone formation"],"prefix":"10.1111","volume":"58","author":[{"given":"Keigo","family":"Nakamura","sequence":"first","affiliation":[{"name":"Department of Operative Dentistry, Endodontology and Periodontology Matsumoto Dental University  Shiojiri Nagano Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6511-9999","authenticated-orcid":false,"given":"Masanori","family":"Koide","sequence":"additional","affiliation":[{"name":"Division of Hard Tissue Research, Institute for Oral Science Matsumoto Dental University  Shiojiri Nagano Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Division of Hard Tissue Research, Institute for Oral Science Matsumoto Dental University  Shiojiri Nagano Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teruhito","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Division of Hard Tissue Research, Institute for Oral Science Matsumoto Dental University  Shiojiri Nagano Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Division of Hard Tissue Research, Institute for Oral Science Matsumoto Dental University  Shiojiri Nagano Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisataka","family":"Yasuda","sequence":"additional","affiliation":[{"name":"Bioindustry Division Oriental Yeast Co., Ltd.  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