{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T15:49:22Z","timestamp":1789573762589,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T00:00:00Z","timestamp":1698278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2019MH003"],"award-info":[{"award-number":["ZR2019MH003"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>Periodontitis is a chronic inflammatory disease linked to pyroptosis, an inflammatory cell death process. Macrophages are essential for maintaining microenvironment homeostasis, which is crucial for periodontal health. This study explores the mechanisms underlying the relationship between macrophage pyroptosis and periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Expression of the pyroptosis marker gasdermin E (GSDME) and the macrophage surface marker CD68 was examined by immunofluorescence double staining in healthy and periodontitis gingival tissues. In an in\u00a0vitro pyroptosis model, RAW264.7 cells were irritated using <jats:italic>Porphyromonas gingivalis<\/jats:italic>\u2013lipopolysaccharide (<jats:italic>P. gingivalis<\/jats:italic>\u2013LPS) after treatment with either a nuclear factor kappa\u2010B (NF\u2010\u03baB) agonist or inhibitor. The mRNA and protein levels of NF\u2010\u03baB, caspase\u20103, GSDME, and interleukin\u20101\u03b2 (IL\u20101\u03b2) were evaluated through qRT\u2010PCR, western blotting, and ELISA techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>GSDME and CD68 were heavily elevated in inflamed gingival tissues compared to healthy tissues and co\u2010localized in the same region. Furthermore, exposure to <jats:italic>P. gingivalis<\/jats:italic>\u2013LPS resulted in a significant upregulation of NF\u2010\u03baB, caspase\u20103, GSDME, and IL\u20101\u03b2 at both the mRNA and protein levels in RAW264.7 cells. NF\u2010\u03baB agonist or inhibitor pretreatment enhanced or inhibited these effects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>GSDME\u2010mediated macrophage pyroptosis is implicated in periodontitis. Based on in\u00a0vitro experiments, <jats:italic>P. gingivalis<\/jats:italic>\u2011LPS causes pyroptosis in RAW264.7 cells through the caspase\u20103\/GSDME pathway. Furthermore, NF\u2010\u03baB regulates this pyroptotic pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13197","type":"journal-article","created":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T06:05:20Z","timestamp":1698386720000},"page":"140-150","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Caspase\u20103 and gasdermin E mediate macrophage pyroptosis in periodontitis"],"prefix":"10.1111","volume":"59","author":[{"given":"Xiangru","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Periodontology The Affiliated Hospital of Qingdao University  Qingdao China"},{"name":"School of Stomatology Qingdao University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Periodontology The Affiliated Hospital of Qingdao University  Qingdao China"},{"name":"School of Stomatology Qingdao 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