{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T21:45:29Z","timestamp":1793828729964,"version":"4.6.1"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:00:00Z","timestamp":1700006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to evaluate the role of miR\u2010150\u20105p in the onset and progression of periodontitis, and reveal the potential molecular mechanism underlying its function and to explore a novel biomarker for periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Periodontitis is the leading cause of tooth loss in adults, emphasizing the need for a biomarker to improve its early detection and prevention. The association of miR\u2010150\u20105p with diseases related to <jats:italic>Fuscobacterium nucleatum<\/jats:italic> implies its potential involvement in periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression of miR\u2010150\u20105p in the saliva of patients with periodontitis (<jats:italic>n<\/jats:italic>\u2009=\u200977) and healthy individuals (<jats:italic>n<\/jats:italic>\u2009=\u200943) was assessed by PCR. Human gingival fibroblasts (HGFs) were induced with an osteogenic culture medium. The regulatory effect of miR\u2010150\u20105p on the proliferation and migration of HGFs was assessed by CCK8 and transwell assays. Osteogenic differentiation was estimated based on the expression of corresponding factors through western blotting, and the inflammatory response was evaluated by measuring the levels of pro\u2010inflammatory cytokines using ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significant upregulation of miR\u2010150\u20105p was observed in patients with periodontitis, which sensitively distinguished them and was closely associated with the severity and periodontal index of the condition. In HGFs, osteogenic induction (OI) resulted in increased miR\u2010150\u20105p levels, which negatively regulated the expression of AIFM2. Silencing miR\u2010150\u20105p significantly attenuated OI\u2010induced suppression of proliferation and migration of HGFs. The silencing also alleviated inflammation and osteogenic differentiation, which was reversed upon AIFM2 knockdown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Upregulated miR\u2010150\u20105p in periodontitis served as a diagnostic biomarker, indicating the occurrence and aggravation of disease condition. Silencing miR\u2010150\u20105p inhibited the osteogenic differentiation and inflammation of HGFs by negatively modulating AIFM2.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13205","type":"journal-article","created":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T21:14:25Z","timestamp":1700082865000},"page":"187-194","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Salivary <scp>miR<\/scp>\u2010150\u20105p as an indicator of periodontitis severity and regulator of human periodontal ligament fibroblast behavior by targeting <scp>AIFM2<\/scp>"],"prefix":"10.1111","volume":"59","author":[{"given":"Jing","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Orthodontics Affiliated Stomatological Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuejing","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Stomatology The Second Affiliated Hospital of FMU  Quanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengcheng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Affiliated Stomatological Hospital Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12221"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12258"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13189"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-011-2342-y"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0034-98872019000600762"},{"issue":"5","key":"e_1_2_10_7_1","first-page":"12","article-title":"Efficacy of nonsurgical periodontal therapy for treatment of 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