{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:32:42Z","timestamp":1790839962641,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T00:00:00Z","timestamp":1705363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010253","name":"Secretar\u00eda de Ciencia y T\u00e9cnica, Universidad de Buenos Aires","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010253","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The objective of the study was to evaluate the expression of oxytocin receptors in normal and inflamed gingiva, as well as the effects of systemic administration of oxytocin in bone loss and gum inflammatory mediators in a rat model of experimental periodontitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background Data<\/jats:title><jats:p>Current evidence supports the hypothesis of a disbalance between the oral microbiota and the host's immune response in the pathogenesis of periodontitis. Increased complexity of the microbial biofilm present in the periodontal pocket leads to local production of nitrogen and oxygen\u2010reactive species, cytokines, chemokines, and other proinflammatory mediators which contribute to periodontal tissue destruction and bone loss. Oxytocin has been suggested to participate in the modulation of immune and inflammatory processes. We have previously shown that oxytocin, nitric oxide, and endocannabinoid system interact providing a mechanism of regulation for systemic inflammation. Here, we aimed at investigating not only the presence and levels of expression of oxytocin receptors on healthy and inflamed gingiva, but also the effects of oxytocin treatment on alveolar bone loss, and systemic and gum expression of inflammatory mediators involved in periodontal tissue damage using ligature\u2010induced periodontitis. Therefore, anti\u2010inflammatory strategies oriented at modulating the host's immune response could be valuable adjuvants to the main treatment of periodontal disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used an animal model of ligature\u2010induced periodontitis involving the placement of a linen thread (Barbour flax 100% linen suture, No. 50; size 2\/0) ligature around the neck of first lower molars of adult male rats. The ligature was left in place during the entire experiment (7\u2009days) until euthanasia. Animals with periodontitis received daily treatment with oxytocin (OXT, 1000\u2009\u03bcg\/kg, sc.) or vehicle and\/or atosiban (3\u2009mg\/kg, sc.), an antagonist of oxytocin receptors. The distance between the cement\u2013enamel junction and the alveolar bone crest was measured in stained hemimandibles in the long axis of both buccal and lingual surfaces of both inferior first molars using a caliper. TNF\u2010\u03b1 levels in plasma were determined using specific rat enzyme\u2010linked immunosorbent assays (ELISA). OXT receptors, IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1 expression were determined in gingival tissues by semiquantitative or real\u2010time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We show that oxytocin receptors are expressed in normal and inflamed gingival tissues in male rats. We also show that the systemic administration of oxytocin prevents the experimental periodontitis\u2010induced increased gum expression of oxytocin receptors, TNF\u2010\u03b1, IL\u20106, and IL\u20101\u03b2 (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Furthermore, we observed a reduction in bone loss in rats treated with oxytocin in our model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results demonstrate that oxytocin is a novel and potent modulator of the gingival inflammatory process together with bone loss preventing effects in an experimental model of ligature\u2010induced periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13212","type":"journal-article","created":{"date-parts":[[2024,1,16]],"date-time":"2024-01-16T08:54:08Z","timestamp":1705395248000},"page":"280-288","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Oxytocin alleviates periodontitis in adult rats"],"prefix":"10.1111","volume":"59","author":[{"given":"Ganna","family":"Dmytrenko","sequence":"first","affiliation":[{"name":"Facultad de Odontolog\u00eda, C\u00e1tedra de Fisiolog\u00eda Universidad de Buenos Aires  Buenos Aires 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