{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T22:25:37Z","timestamp":1780525537314,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T00:00:00Z","timestamp":1702598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2504200"],"award-info":[{"award-number":["2022YFC2504200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U22A20314"],"award-info":[{"award-number":["U22A20314"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071072"],"award-info":[{"award-number":["82071072"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing","doi-asserted-by":"publisher","award":["CSTB2022NSCQ\u2010LZX0004"],"award-info":[{"award-number":["CSTB2022NSCQ\u2010LZX0004"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study sought to explore the role of developmental endothelial locus\u20101 (DEL\u20101) in osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and investigate the therapeutic effect of DEL\u20101 in ligature\u2010induced experimental periodontitis with type 2 diabetes mellitus (T2DM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>T2DM is a significant risk factor for periodontitis. Treatment modalities for periodontitis with T2DM are being explored. DEL\u20101 is a versatile protein that can modulate the different stages of inflammatory diseases including periodontitis. The direct effect of DEL\u20101 on osteogenic differentiation of PDLSCs in periodontitis with T2DM is poorly understood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Primary hPDLSCs were isolated from periodontal ligament tissue and identified by flow cytometry. In osteogenesis experiments, alkaline phosphatase (ALP), Alizarin Red staining and western blot were used to assess the osteogenic effect of DEL\u20101 on hPDLSCs in high glucose and inflammation environments. The mouse model of ligature\u2010induced experimental periodontitis was established. H&amp;E and Masson's trichrome staining were used to assess the change of periodontal tissue after local periodontal injection of DEL\u20101. Immunohistochemical staining was used to evaluate osteogenic\u2010related protein expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>hPDLSCs expressed mesenchymal stem cell (MSC)\u2010specific surface markers and were negative for hematopoietic cell surface markers. hPDLSCs had the potential for multidirectional differentiation. DEL\u20101 could enhance the osteogenic differentiation of hPDLSCs in high glucose and inflammation environments, although it did not return to the control level. Histological staining showed that DEL\u20101 contributed to alveolar bone regeneration and osteogenic\u2010related protein expression, but the degree of improvement in T2DM mice was lower than in non\u2010T2DM mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In summary, we demonstrated that DEL\u20101 could promote osteogenic differentiation of hPDLSCs in high glucose and inflammation environment and rescue alveolar bone loss in experimental periodontitis with T2DM, which could provide a novel therapeutic target for periodontitis with T2DM.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13219","type":"journal-article","created":{"date-parts":[[2023,12,16]],"date-time":"2023-12-16T04:36:05Z","timestamp":1702701365000},"page":"355-365","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Developmental endothelial locus\u20101 promotes osteogenic differentiation and alveolar bone regeneration 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