{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T20:08:20Z","timestamp":1792267700486,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T00:00:00Z","timestamp":1714694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective and Background<\/jats:title><jats:p>This research aimed to examine the role of C\u2010X\u2010C motif chemokine ligand 5 (CXCL5) and C\u2010X\u2010C motif chemokine ligand 8 (CXCL8; also known as IL\u20108) in neutrophilic inflammation triggered by peri\u2010implantitis and to shed light on the underlying mechanisms that link them to the development of this condition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials<\/jats:title><jats:p>This study included 40 patients who visited the Department of Periodontology at Kyungpook University Dental Hospital. They were divided into two groups based on their condition: healthy implant (HI) group (<jats:italic>n<\/jats:italic>\u2009=\u200920) and peri\u2010implantitis (PI) group (<jats:italic>n<\/jats:italic>\u2009=\u200920). Biopsy samples of PI tissue were collected from the patients under local anesthesia. HI tissue was obtained using the same method during the second implant surgery. To construct libraries for control and test RNAs, the QuantSeq 3\u2032 mRNA\u2010Seq Library Prep Kit (Lexogen, Inc., Austria) was used according to the manufacturer's instructions. Samples were pooled based on representative cytokines obtained from RNA sequencing results and subjected to Reverse transcription\u2010quantitative polymerase chain reaction (RT\u2010qPCR). Hematoxylin and eosin staining, and immunohistochemistry (IHC) analysis were performed to visually assess expression levels and analyze tissue histology. Student's <jats:italic>t<\/jats:italic>\u2010test was employed to conduct statistical analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Initially, heatmaps were used to examine gene expression variations between the HI and PI groups based on the results of RNA sequencing. Notably, among various cytokines, CXCL5 and CXCL8 had the highest expression levels in the PI group compared with the HI group, and they are known to be associated with inflammatory responses. In the gingival tissues, the expression of genes encoding cytokines such as interleukin (IL)\u20101\u03b2, tumor necrosis factor\u2010alpha (TNF)\u2010\u03b1, interleukin (IL)\u20106, and CXCL5\/CXCL8 was assessed via RT\u2010qPCR. The mRNA expression level of CXCL5\/CXCL8 significantly increased in the PI group compared with the HI group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.045). Contrarily, the mRNA expression level of interleukin 36 receptor antagonist (IL36RN) significantly decreased (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.008). IHC enabled examination of the distribution and intensity of CXCL5\/CXCL8 protein expression within the tissue samples. Specifically, increased levels of CXCL5\/CXCL8 promote inflammatory responses, cellular proliferation, migration, and invasion within the peri\u2010implant tissues. These effects are mediated through the activation of the PI3K\/Akt\/NF\u2010\u03baB signaling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study found that the PI sites had higher gene expression level of CXCL8\/CXCL5 in the soft tissue than HI sites, which could help achieve more accurate diagnosis and treatment planning.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13230","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T08:39:14Z","timestamp":1714725554000},"page":"698-711","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["<scp>CXCL5<\/scp>\/<scp>CXCL8<\/scp> induces neutrophilic inflammation in peri\u2010implantitis"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5473-1230","authenticated-orcid":false,"given":"Seong\u2010Eun","family":"Park","sequence":"first","affiliation":[{"name":"Department of Periodontology Kyungpook National University School of Dentistry  Daegu Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2402-2753","authenticated-orcid":false,"given":"Kanghyun","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Biotechnology, Research Institute for Innovative Animal Science Kyungpook National University  Sangju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8434-0915","authenticated-orcid":false,"given":"Eungyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Biotechnology, Research Institute for Innovative Animal Science Kyungpook National University  Sangju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7301-4444","authenticated-orcid":false,"given":"Chae Yeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Biotechnology, Research Institute for Innovative Animal Science Kyungpook National University  Sangju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2701-4854","authenticated-orcid":false,"given":"Sung\u2010Min","family":"Hwang","sequence":"additional","affiliation":[{"name":"Department of Periodontology Kyungpook National University School of Dentistry  Daegu Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0291-6114","authenticated-orcid":false,"given":"Jae\u2010Mok","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Periodontology Kyungpook National University School of Dentistry  Daegu Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3515-3854","authenticated-orcid":false,"given":"Jo\u2010Young","family":"Suh","sequence":"additional","affiliation":[{"name":"Department of Periodontology Kyungpook National University School of Dentistry  Daegu Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9049-6908","authenticated-orcid":false,"given":"Youngkyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Institute for Hard Tissue and Bone Regeneration Kyungpook National University School of Dentistry  Daegu Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6650-7734","authenticated-orcid":false,"given":"Myoung Ok","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Biotechnology, Research Institute for Innovative Animal Science Kyungpook National University  Sangju Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2793-7667","authenticated-orcid":false,"given":"Yong\u2010Gun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Periodontology Kyungpook National University School of Dentistry  Daegu Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,3]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12954"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2008.01275.x"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2015.140603"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12354"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2007.01463.x"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-7757-2018-0316"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/clr.12052"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fdmed.2021.687638"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1785"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02182"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.13994"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1998.69.10.1139"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1431-6"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00897.x"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12957"},{"issue":"4","key":"e_1_2_12_17_1","first-page":"416","article-title":"A prognosis system for periimplant diseases","volume":"24","author":"Decker AM","year":"2015","journal-title":"Implant Dent"},{"issue":"1","key":"e_1_2_12_18_1","first-page":"49","article-title":"Ginger\u2010derived compounds exert in\u00a0vivo and in\u00a0vitro anti\u2010asthmatic effects by inhibiting the T\u2010helper 2 cell\u2010mediated allergic response","volume":"23","author":"Eungyung K","year":"2022","journal-title":"Exp Ther Med"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.59.1.24"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.6.1355"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)47243-2"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117414"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm11195817"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering10030356"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgt397"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.07.012"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a001651"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2011.01.006"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0000000000000059"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.118.255893"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510376402"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.13230","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T11:04:40Z","timestamp":1722337480000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.13230"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,3]]},"references-count":31,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,8]]}},"alternative-id":["10.1111\/jre.13230"],"URL":"https:\/\/doi.org\/10.1111\/jre.13230","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,3]]},"assertion":[{"value":"2023-10-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}