{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:10:18Z","timestamp":1787771418124,"version":"build-2784847793"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81991500"],"award-info":[{"award-number":["81991500"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81991503"],"award-info":[{"award-number":["81991503"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["18ZR1422400"],"award-info":[{"award-number":["18ZR1422400"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>In order to evaluate the effect of methacrylated hyaluronic acid (HAMA) hydrogels containing the recombinant human amelogenin (rhAm) in\u00a0vitro and in\u00a0vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>The ultimate goal in treating periodontal disease is to control inflammation and achieve regeneration of periodontal tissues. In recent years, methacrylated hyaluronic acid (HAMA) containing recombinant human amyloid protein (rhAm) has been widely used as a new type of biomaterial in tissue engineering and regenerative medicine. However, there is a lack of comprehensive research on the periodontal regeneration effects of this hydrogel. This experiment aims to explore the application of photoresponsive recombinant human amelogenin\u2010loaded hyaluronic acid hydrogel for periodontal tissue regeneration and provide valuable insights into its potential use in this field.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The effects of rhAm\u2010HAMA hydrogel on the proliferation of human periodontal ligament cells (hPDLCs) were assessed using the CCK\u20108 kit. The osteogenic differentiation of hPDLCs was evaluated through ALP staining and real\u2010time PCR. Calvarial parietal defects were created in 4\u2010week\u2010old Sprague Dawley rats and implanted with deproteinized bovine bone matrix in different treatment groups. The animals were euthanized after 4 and 8\u2009weeks of healing. The bone volume of the defect was observed by micro\u2010CT and histological analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Stimulating hPDLCs with rhAm\u2010HAMA hydrogel did not significantly affect their proliferation (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). ALP staining and real\u2010time PCR results demonstrated that the rhAm\u2010HAMA group exhibited a significant upregulation of osteoclastic gene expression (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Micro\u2010CT results revealed a significant increase in mineralized tissue volume fraction (MTV\/TV%), trabecular bone number (Tb.N), and mineralized tissue density (MTD) of the bone defect area in the rhAm\u2010HAMA group compared to the other groups (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). The results of hematoxylin and eosin staining and Masson staining at 8\u2009weeks post\u2010surgery further supported the results of the micro\u2010CT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results of this study indicate that rhAm\u2010HAMA hydrogel could effectively promote the osteogenic differentiation of hPDLCs and stabilize bone substitutes in the defects that enhance the bone regeneration in\u00a0vivo.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13235","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T06:59:20Z","timestamp":1710399560000},"page":"589-598","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["A photoresponsive recombinant human amelogenin\u2010loaded hyaluronic acid hydrogel promotes bone regeneration"],"prefix":"10.1111","volume":"59","author":[{"given":"Tung\u2010Liang","family":"Hsia","sequence":"first","affiliation":[{"name":"Department of Periodontology, Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhikai","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9955-7375","authenticated-orcid":false,"given":"Yiru","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Shu","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research Center for Oral Diseases  Shanghai China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.3241"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma14247588"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12156"},{"issue":"1","key":"e_1_2_10_5_1","first-page":"29","article-title":"Localized ridge augmentation using guided bone regeneration. 1. 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