{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:12:58Z","timestamp":1792109578702,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T00:00:00Z","timestamp":1708992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82201072"],"award-info":[{"award-number":["82201072"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The aim of this study was to investigate the association between autoinducer\u20102 (AI\u20102) of oral microbial flora and the alveolar bone destruction in periodontitis to determine if AI\u20102 may have the potential that monitor periodontitis and predict bone loss.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Plaque biofilm was the initiating factor of periodontitis and the essential factor of periodontal tissue destruction. The formation of biofilms depended on the complex regulation of the quorum sensing (QS) system, in which bacteria could sense changes in surrounding bacterial density by secreting the autoinducer (AI) to regulate the corresponding physiological function. Most oral bacteria also communicated with each other to form biofilms administrating the QS system, which implied that the QS system of periodontal pathogens was related to periodontitis, but the specific relationship was unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      We collected the gingival crevicular fluid (GCF) samples and measured the concentration of AI\u20102 in samples using the\n                      <jats:italic>Vibrio harveyi<\/jats:italic>\n                      BB180 bioluminescent\u2010reporter system. To explore the interaction between AI\u20102 and bone metabolism, we utilized AI\u20102 purified from\n                      <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                      to investigate the impact of\n                      <jats:italic>F. nucleatum<\/jats:italic>\n                      AI\u20102 on osteoclast differentiation. Moreover, we constructed murine periodontitis models and multi\u2010species biofilm models to study the association between AI\u20102 and periodontal disease progression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The AI\u20102 concentration in GCF samples increased along with periodontal disease progression (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.0001).\n                      <jats:italic>F. nucleatum<\/jats:italic>\n                      AI\u20102 promoted osteoclast differentiation in a dose\u2010dependent manner. In the periodontitis mice model, the CEJ\u2010ABC distance in the\n                      <jats:italic>F. nucleatum<\/jats:italic>\n                      AI\u20102 treatment group was higher than that in the simple ligation group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.01), and the maxilla of the mice in the group exhibited significantly lower BMD and BV\/TV values (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      We demonstrated that the AI\u20102 concentration varied with the alveolar bone destruction in periodontitis, and it may have the potential for screening periodontitis.\n                      <jats:italic>F. nucleatum<\/jats:italic>\n                      AI\u20102 promoted osteoclast differentiation in a dose\u2010dependent manner and aggravated bone loss.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jre.13247","type":"journal-article","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T06:59:25Z","timestamp":1709017165000},"page":"576-588","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Autoinducer\u20102 produced by oral microbial flora and alveolar bone loss in periodontitis"],"prefix":"10.1111","volume":"59","author":[{"given":"Cheng","family":"Li","sequence":"first","affiliation":[{"name":"Department of Periodontics Affiliated Stomatological Hospital of Nanjing Medical University  Nanjing China"},{"name":"Jiangsu Key Laboratory of Oral Disease Nanjing Medical University  Nanjing China"},{"name":"Jiangsu Province Engineering Research Center of Stomatological Translational Medicine  Nanjing 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