{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:14:22Z","timestamp":1791245662884,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T00:00:00Z","timestamp":1714694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Prevention of periodontal bone resorption triggered by <jats:italic>Porphyromonas gingivalis<\/jats:italic> (<jats:italic>P. gingivalis<\/jats:italic>) is crucial for dental stability. Capsaicin, known as the pungent ingredient of chili peppers, can activate key signaling molecules involved in osteogenic process. However, the effect of capsaicin on osteogenesis of periodontal ligament stem cells (PDLSCs) under inflammation remains elusive.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>P. gingivalis<\/jats:italic> culture suspension was added to mimic the inflammatory status after capsaicin pretreatment. The effects of capsaicin on the osteogenesis of PDLSCs, as well as mitochondrial morphology, Ca<jats:sup>2+<\/jats:sup> level, reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and osteogenesis\u2010regulated protein expression levels were analyzed. Furthermore, a mouse experimental periodontitis model was established to evaluate the effect of capsaicin on alveolar bone resorption and the expression of osteogenesis\u2010related proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Under <jats:italic>P. gingivalis<\/jats:italic> stimulation, capsaicin increased osteogenesis of PDLSCs. Not surprisingly, capsaicin rescued the damage to mitochondrial morphology, decreased the concentration of intracellular Ca<jats:sup>2+<\/jats:sup> and ROS, enhanced MMP and activated phosphatidylinositol 3\u2010kinase (PI3K)\/protein kinase B (AKT)\/mammalian target of rapamycin (mTOR) pathway. The in\u00a0vivo results showed that capsaicin significantly attenuated alveolar bone loss and augmented the expression of bone associated proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Capsaicin increases osteogenesis of PDLSCs under inflammation and reduces alveolar bone resorption in mouse experimental periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13252","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T08:38:49Z","timestamp":1714725529000},"page":"798-811","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Capsaicin attenuates <i>Porphyromonas gingivalis<\/i>\u2010suppressed osteogenesis of periodontal ligament stem cells via regulating mitochondrial function and activating <scp>PI3K<\/scp>\/<scp>AKT<\/scp>\/<scp>mTOR<\/scp> pathway"],"prefix":"10.1111","volume":"59","author":[{"given":"Weijia","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Periodontology &amp; Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration &amp; Shandong Provincial Clinical Research Center for Oral Diseases  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Periodontology &amp; Endodontics, School and Hospital of Stomatology, Cheeloo College of Medicine Shandong University &amp; Shandong Key Laboratory of Oral Tissue Regeneration &amp; Shandong Engineering Research Center of Dental Materials and Oral Tissue Regeneration &amp; Shandong Provincial Clinical Research Center for Oral Diseases  Jinan 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