{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:27:45Z","timestamp":1788910065734,"version":"build-2803163510"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T00:00:00Z","timestamp":1714694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objective<\/jats:title>\n                    <jats:p>\n                      Osteoporosis is associated with bone microarchitecture alterations, and the depletion of estrogen during menopause is a major contributing factor to its development. The literature highlights the noteworthy role of gut microbiota in bone metabolism, particularly in the progression of osteoporosis. Periodontal disease leads to alveolar bone loss, which may be influenced by estrogen deficiency, and this mechanism is intricately associated with an imbalance in systemic microbiota. The aim of this study was to evaluate the effects of\n                      <jats:italic>Bifidobacterium animalis<\/jats:italic>\n                      subsp.\n                      <jats:italic>lactis<\/jats:italic>\n                      HN019 (\n                      <jats:italic>B.\u2009lactis<\/jats:italic>\n                      HN019) and\n                      <jats:italic>Lacticaseibacillus casei<\/jats:italic>\n                      01 (\n                      <jats:italic>L.\u2009casei<\/jats:italic>\n                      01) administrations on an osteoporosis animal model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Thirty\u2010three female rats were randomly divided into three groups: control (C\u2010OVX), C\u2010OVX\u2010HN019 and C\u2010OVX\u2010LC01. All animals were ovariectomized. In groups C\u2010OVX\u2010HN019 and C\u2010OVX\u2010LC01, the probiotics were administered for 4\u2009months. All animals were euthanized after 16\u2009weeks from ovariectomy. Microtomographic, histopathological and immunohistochemical examinations were conducted on periodontal tissues, whereas histomorphometry, histopathological and immunohistochemical analyses were carried out on the intestine. The levels of estradiol were assessed in blood using an immunoenzymatic assay. The data were subjected to statistical analyses (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The C\u2010OVX\u2010LC01 group exhibited a significant reduction in alveolar bone porosity and an increase in connective tissue density compared to C\u2010OVX (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05). The C\u2010OVX\u2010HN019 and C\u2010OVX\u2010LC01 groups presented reduced expression of TRAP and RANKL compared to the C\u2010OVX (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05). The C\u2010OVX group presented villi defects, mild neutrophil infiltration, decrease in both villous height and intestinal crypts and reduced expression of intestinal junctional epithelium markers e\u2010cadherin and claudin 01 compared to C\u2010OVX\u2010HN019 and C\u2010OVX\u2010LC01 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05). The C\u2010OVX group had lower estradiol levels than C\u2010OVX\u2010HN019 and C\u2010OVX\u2010LC01 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The probiotic therapy promoted a reduction in alveolar bone destruction and intestinal permeability as well as an increase in estradiol levels in ovariectomized rats. Specifically, the probiotic strain\n                      <jats:italic>Lacticaseibacillus casei<\/jats:italic>\n                      01 exhibited greater effectiveness compared to\n                      <jats:italic>Bifidobacterium animalis<\/jats:italic>\n                      subsp.\n                      <jats:italic>lactis<\/jats:italic>\n                      HN019, indicating strain\u2010dependent outcomes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jre.13256","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T04:30:51Z","timestamp":1714710651000},"page":"758-770","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Probiotics enhance alveolar bone microarchitecture, intestinal morphology and estradiol levels in osteoporotic animals"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1161-3999","authenticated-orcid":false,"given":"R. 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