{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T12:06:22Z","timestamp":1791806782274,"version":"4.3.4"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["110\u20102628\u2010B\u2010002\u2010036"],"award-info":[{"award-number":["110\u20102628\u2010B\u2010002\u2010036"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005762","name":"National Taiwan University Hospital","doi-asserted-by":"publisher","award":["110\u2010S5072"],"award-info":[{"award-number":["110\u2010S5072"]}],"id":[{"id":"10.13039\/501100005762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>The microbial profiles of peri\u2010implantitis and periodontitis (PT) are inconclusive. The controversies mainly arise from the differences in sampling sites, targeted gene fragment, and microbiome analysis techniques. The objective of this study was to explore the microbiomes of peri\u2010implantitis (PI), control implants (CI), PT and control teeth (CT), and the microbial change of PI after nonsurgical treatment (PIAT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010two patients diagnosed with both PT and peri\u2010implantitis were recruited. Clinical periodontal parameters and radiographic bone levels were recorded. In each patient, the subgingival and submucosal plaque samples were collected from sites with PI, CI, PT, CT, and PIAT. Microbiome diversity was analyzed by high\u2010throughput amplicon sequencing using full\u2010length of 16S rRNA gene by next generation sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The 16S rRNA gene sequencing analysis revealed 512 OTUs in oral microbiome and 377 OTUs reached strain levels. The PI and PT groups possessed their own unique core microbiome. <jats:italic>Treponema denticola<\/jats:italic> was predominant in PI with probing depth of 8\u201310\u2009mm. Interestingly, <jats:italic>Thermovirga lienii DSM 17291<\/jats:italic> and <jats:italic>Dialister invisus DSM 15470<\/jats:italic> were found to associate with PI. Nonsurgical treatment for peri\u2010implantitis did not significantly alter the microbiome, except <jats:italic>Rothia aeria<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study suggests <jats:italic>Treponemas<\/jats:italic> species may play a pivotal role in peri\u2010implantitis. Nonsurgical treatment did not exert a major influence on the peri\u2010implantitis microbiome in short\u2010term follow\u2010up. PT and peri\u2010implantitis possess the unique microbiome profiles, and different therapeutic strategies may be suggested in the future.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13269","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T17:33:22Z","timestamp":1715189602000},"page":"657-668","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Microbiome of periodontitis and peri\u2010implantitis before and after therapy: Long\u2010read 16S rRNA gene amplicon sequencing"],"prefix":"10.1111","volume":"59","author":[{"given":"Pei\u2010Shiuan","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Dentistry, National Taiwan University Hospital and Graduate Institute of Clinical Dentistry National Taiwan University  Taipei 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