{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T12:31:21Z","timestamp":1789561881163,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,5,25]],"date-time":"2024-05-25T00:00:00Z","timestamp":1716595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2506300"],"award-info":[{"award-number":["2023YFC2506300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012542","name":"Sichuan Province Science and Technology Support Program","doi-asserted-by":"publisher","award":["2023NSFSC0553"],"award-info":[{"award-number":["2023NSFSC0553"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      This study sought to explore the impact of\n                      <jats:italic>Fusobacterium nucleatum<\/jats:italic>\n                      on hepatic steatosis in apolipoprotein E (ApoE) knockout (KO) mice induced by a high\u2010fat diet (HFD) and elucidate the underlying mechanism.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      ApoE KO mice, on a HFD, received\n                      <jats:italic>F.\u2009nucleatum<\/jats:italic>\n                      oral inoculation every other day. After 24\u2009weeks, body weight, liver weight, and liver index were assessed. Serum biochemistry and pro\u2010inflammatory factors in serum and liver were analyzed. The histopathology of right maxilla and live were performed. Oil red O, immunohistochemistry, and immunofluorescence staining for the liver were conducted. Myeloperoxidase (MPO) activity, apoptosis, lipid reactive oxygen species (ROS), ROS, lipid peroxides, and hepatic lipids were also evaluated. Liver inflammation, fibrosis, de novo lipogenesis (DNL)\u2010related molecule, and Nrf2\/Keap1\u2010related signaling molecule gene\/protein expression were determined by real\u2010time PCR (RT\u2010PCR) and\/or Western blot (WB) analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      HFD\u2010fed ApoE KO mice infected by\n                      <jats:italic>F.\u2009nucleatum<\/jats:italic>\n                      demonstrated significant changes, including increased body and liver weight, elevated proinflammatory factors and lipids in serum and liver, as well as neutrophil infiltration, fibrosis, apoptosis, oxidative stress, and lipid peroxidation in the liver. Additionally,\n                      <jats:italic>F.\u2009nucleatum<\/jats:italic>\n                      stimulates hepatic lipid accumulation and activates de novo lipogenesis (DNL), while simultaneously suppressing the Nrf2\/Keap1 antioxidant pathway.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      In conclusion, our study reveals that oral inoculation of\n                      <jats:italic>F.\u2009nucleatum<\/jats:italic>\n                      might promote hepatic steatosis by inhibiting Nrf2\/Keap1 pathway.\n                    <\/jats:p>\n                  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