{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T05:33:21Z","timestamp":1739338401147,"version":"3.37.0"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,5,19]],"date-time":"2024-05-19T00:00:00Z","timestamp":1716076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>We investigated the in\u00a0vitro effect of <jats:italic>Limosilactobacillus reuteri<\/jats:italic> DSM 17938 supernatant on the inflammatory response of human gingival fibroblasts (HGF) challenged by lipopolysaccharide (LPS) or elevated glucose levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HGF were exposed to LPS (1\u2009\u03bcg\/mL), glucose (5, 12\u2009mM or 25\u2009mM), and dilutions of supernatant prepared from <jats:italic>L. reuteri<\/jats:italic> DSM 17938 (0.5\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>, 1.0\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>, 2.5\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>, and 5.0\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>\u2009CFU\/mL). After 24\u2009h cell viability and levels of cytokines (<jats:italic>IL\u20101\u03b2<\/jats:italic>, <jats:italic>IL\u20106<\/jats:italic> and <jats:italic>IL\u20108<\/jats:italic>) and <jats:italic>TLR\u20102<\/jats:italic> were determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>None of the tested <jats:italic>L. reuteri<\/jats:italic> (DSM 17938) supernatant concentrations reduced the viability of HGF. Supernatant concentrations (2.5\u2009\u00d7\u200910<jats:sup>7<\/jats:sup> and 5\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>\u2009CFU\/mL) significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05) decreased the production of <jats:italic>IL\u20101\u03b2<\/jats:italic>, <jats:italic>IL\u20106<\/jats:italic>, <jats:italic>IL\u20108<\/jats:italic>, and <jats:italic>TLR\u2010<\/jats:italic>2 in the presence of LPS. In contrast, inflammatory markers were not reduced by <jats:italic>L. reuteri<\/jats:italic> supernatant in the presence of glucose. Glucose concentrations of 12\u2009mM and 24\u2009mM still lead to an elevated production of the investigated biochemical mediators.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>While <jats:italic>L. reuteri<\/jats:italic> (DSM 17938) supernatant attenuates the inflammatory response of HGF to LPS in a dose\u2010dependent manner, elevated glucose levels suppress this action. These in\u00a0vitro results support the overall anti\u2010inflammatory efficacy of <jats:italic>L. reuteri<\/jats:italic> supplementation in plaque\u2010associated periodontal inflammations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13290","type":"journal-article","created":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T03:45:03Z","timestamp":1716176703000},"page":"974-981","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Limosilactobacillus reuteri<\/i> supernatant attenuates inflammatory responses of human gingival fibroblasts to <scp>LPS<\/scp> but not to elevated glucose levels"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-6855-5488","authenticated-orcid":false,"given":"T. M.","family":"Janson","sequence":"first","affiliation":[{"name":"Division of Periodontology and Peri\u2010implant Diseases, Clinic of Conservative and Preventive Dentistry, Center for Dental Medicine University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7732-2118","authenticated-orcid":false,"given":"L. L.","family":"Ramenzoni","sequence":"additional","affiliation":[{"name":"Division of Periodontology and Peri\u2010implant Diseases, Clinic of Conservative and Preventive Dentistry, Center for Dental Medicine University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3317-0770","authenticated-orcid":false,"given":"C. R.","family":"Hatz","sequence":"additional","affiliation":[{"name":"Division of Periodontology and Peri\u2010implant Diseases, Clinic of Conservative and Preventive Dentistry, Center for Dental Medicine University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Schlagenhauf","sequence":"additional","affiliation":[{"name":"Division of Periodontology and Peri\u2010implant Diseases, Clinic of Conservative and Preventive Dentistry, Center for Dental Medicine University of Zurich  Zurich Switzerland"},{"name":"Department of Conservative Dentistry and Periodontology, Center for Oral Health University Hospital Wuerzburg  Wuerzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6141-1263","authenticated-orcid":false,"given":"T.","family":"Attin","sequence":"additional","affiliation":[{"name":"Division of Periodontology and Peri\u2010implant Diseases, Clinic of Conservative and Preventive Dentistry, Center for Dental Medicine University of Zurich  Zurich Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1377-0325","authenticated-orcid":false,"given":"P. 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