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The current investigation compared for the first\u2010time direct effects of oxLDL to a cytokine cocktail of IL\u20101\u00df\/TNF\u2010\u0251\/INF\u2010\u03b3 on gingival mesenchymal stem cells' (G\u2010MSCs) attributes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human third passage G\u2010MSCs, isolated from connective tissue biopsies (<jats:italic>n<\/jats:italic>\u2009=\u20095) and characterized, were stimulated in three groups over 7\u2009days: control group, cytokine group (IL\u20101\u03b2[1\u2009ng\/mL], TNF\u2010\u03b1[10\u2009ng\/mL], IFN\u2010\u03b3[100\u2009ng\/mL]), or oxLDL group (oxLDL [50\u2009\u03bcg\/mL]). Next Generation Sequencing and KEGG pathway enrichment analysis, stemness gene expression (<jats:italic>NANOG\/SOX2\/OCT4A<\/jats:italic>), cellular proliferation, colony\u2010formation, multilinear potential, and altered intracellular pathways were investigated via histochemistry, next\u2010generation sequencing, and RT\u2010qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>G\u2010MSCs exhibited all mesenchymal stem cells' characteristics. oxLDL group and cytokine group displayed no disparities in their stemness markers (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). Next\u2010generation\u2010sequencing revealed altered expression of the TXNIP gene in response to oxLDL treatment compared with controls (<jats:italic>p<\/jats:italic>\u2009=\u2009.04). Following an initial boosting for up to 5\u2009days by inflammatory stimuli, over 14\u2009day, cellular counts [median count \u00d710<jats:sup>\u22125<\/jats:sup> (Q25\/Q75)] were utmost in control \u2013 [2.6607 (2.0804\/4.5357)], followed by cytokine \u2013 [0.0433 (0.0026\/1.4215)] and significantly lowered in the oxLDL group [0.0274 (0.0023\/0.7290); <jats:italic>p<\/jats:italic>\u2009=\u2009.0047]. Osteogenic differentiation [median relative Ca<jats:sup>2+<\/jats:sup> content(Q25\/Q75)] was significantly lower in cytokine \u2013 [0.0066 (0.0052\/0.0105)] compared to oxLDL \u2013 [0.0144 (0.0108\/0.0216)] (<jats:italic>p<\/jats:italic>\u2009=\u2009.0133), with no differences notable for chondrogenic and adipogenic differentiation (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Within the current investigation's limitations, in contrast to cytokine\u2010mediated inflammation, G\u2010MSCs appear to be minimally responsive to oxLDL\u2010mediated metabolic inflammation, with little negative effect on their differentiation attributes and significantly reduced cellular proliferation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13319","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T07:30:55Z","timestamp":1719905455000},"page":"77-89","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of oxidized <scp>LDL<\/scp> versus <scp>IL<\/scp>\u20101\u00df\/<scp>TNF<\/scp>\u2010\u0251\/<scp>INF\u0263<\/scp> on human gingival mesenchymal stem cells properties"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6261-3609","authenticated-orcid":false,"given":"Karim","family":"Fawzy El\u2010Sayed","sequence":"first","affiliation":[{"name":"Clinic for Conservative Dentistry and Periodontology University Hospital of Schleswig\u2010Holstein  Kiel Germany"},{"name":"Oral Medicine and Periodontology Department, Faculty of Dentistry Cairo University  Cairo Egypt"},{"name":"Stem Cells and Tissue Engineering Unit, Faculty of Dentistry Cairo University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Mahlandt","sequence":"additional","affiliation":[{"name":"Institute of Diabetes and Clinical Metabolic Research University Hospital of Schleswig\u2010Holstein  Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Schlicht","sequence":"additional","affiliation":[{"name":"Institute of Diabetes and Clinical Metabolic Research University Hospital of Schleswig\u2010Holstein  Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Enthammer","sequence":"additional","affiliation":[{"name":"Institute of Diabetes and Clinical Metabolic Research University Hospital of Schleswig\u2010Holstein  Kiel Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"T\u00f6lle","sequence":"additional","affiliation":[{"name":"Clinic for Conservative Dentistry and 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