{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:10:42Z","timestamp":1789495842778,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,8,1]],"date-time":"2024-08-01T00:00:00Z","timestamp":1722470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100002050","name":"Delta Dental Foundation","doi-asserted-by":"publisher","award":["AWD010089"],"award-info":[{"award-number":["AWD010089"]}],"id":[{"id":"10.13039\/100002050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of this study is to assess early wound healing expression of local angiogenic biomarkers following connective tissue graft (CTG) at dental implant sites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010eight subjects with single dental implants exhibiting a soft tissue dehiscence were included and randomly treated with CTG, either with coronally advanced flap (CAF) or with tunnel technique (TUN). Peri\u2010implant crevicular fluid (PICF) was collected at the midfacial and midlingual aspect of the implant sites at baseline and at 3, 7, 14, 30, and 90\u2009days after the surgical intervention. The expression of angiogenin (ANG), fibroblast growth factor\u20102 (FGF\u20102), platelet\u2010derived growth factor (PDGF), tissue inhibitor of metalloproteinases\u20102 (TIMP\u20102), and vascular endothelial growth factor (VEGF) was investigated over a period of 3\u2009months. Patient\u2010reported outcomes, clinical measurements, and ultrasonography scans at multiple time points were also evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The longitudinal regression revealed a significant difference in the expression of VEGF and TIMP\u20102 between CAF\u2010 and TUN\u2010treated sites over 3\u2009months (<jats:italic>p<\/jats:italic>\u2009=\u2009.033 and <jats:italic>p<\/jats:italic>\u2009=\u2009.004, respectively), whereas no significant differences were observed for ANG, FGF\u20102 and PDGF between the two groups. At 7\u2009days, a direct correlation was observed between ANG levels and ultrasonographic color velocity in the CAF group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) and between ANG levels and ultrasonographic color power in the TUN group (<jats:italic>p<\/jats:italic>\u2009=\u2009.028). VEGF levels and ultrasonographic mean perfused area of the CTG were significantly correlated at the 7\u2010day time point (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001 for both CAF and TUN). The expression of VEGF at 7\u2009days was directly associated with mucosal thickness gain at 1\u2009year (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001 for both groups). Early TIMP\u20102 expression showed an inverse correlation with time to recovery (<jats:italic>p<\/jats:italic>\u2009=\u2009.002). TIMP\u20102 levels at 3\u2009months exhibited inverse correlations with mean dehiscence coverage (<jats:italic>p<\/jats:italic>\u2009=\u2009.004) and the rate of complete dehiscence coverage (<jats:italic>p<\/jats:italic>\u2009=\u2009.012).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PICF biomarkers can be used to monitor early wound healing events following soft tissue grafting at implant sites. VEGF and TIMP\u20102 showed correlations with the 1\u2010year clinical and volumetric outcomes, as well as with post\u2010operative patient\u2010reported outcomes and Doppler Ultrasonographic tissue perfusion\u2010related parameters.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13328","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T03:29:34Z","timestamp":1722569374000},"page":"206-214","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Characterization of oral biomarkers during early healing at augmented dental implant sites"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4864-3964","authenticated-orcid":false,"given":"Lorenzo","family":"Tavelli","sequence":"first","affiliation":[{"name":"Division of Periodontology, Department of Oral Medicine, Infection, and Immunity Harvard School of Dental Medicine  Boston Massachusetts USA"},{"name":"Center for Clinical Research and Evidence Synthesis in Oral Tissue Regeneration (CRITERION)  Boston Massachusetts USA"},{"name":"School of Dentistry Universidad Catolica de Santiago de Guayaquil (UCSG)  Guayaquil Ecuador"},{"name":"Department of Periodontics &amp; Oral Medicine University of Michigan School of Dentistry  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5347-6577","authenticated-orcid":false,"given":"Shayan","family":"Barootchi","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Department of Oral Medicine, Infection, and Immunity Harvard School of Dental Medicine  Boston Massachusetts USA"},{"name":"Center for Clinical Research and Evidence Synthesis in Oral Tissue Regeneration (CRITERION)  Boston Massachusetts USA"},{"name":"Department of Periodontics &amp; Oral Medicine University of Michigan School of Dentistry  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Vera","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Periodontics &amp; Oral Medicine University of Michigan School of Dentistry  Ann Arbor Michigan USA"},{"name":"Postgraduate Periodontics, Division of Periodontics Columbia University College of Dental Medicine  New York City New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim","family":"Sugai","sequence":"additional","affiliation":[{"name":"Department of Periodontics &amp; Oral Medicine University of Michigan School of Dentistry  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9752-5377","authenticated-orcid":false,"given":"David T.","family":"Wu","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Department of Oral Medicine, Infection, and Immunity Harvard School of Dental Medicine  Boston Massachusetts USA"},{"name":"Harvard John A. Paulson School of Engineering and Applied Sciences Harvard University  Cambridge Massachusetts USA"},{"name":"Wyss Institute for Biologically Inspired Engineering Harvard University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3046-4338","authenticated-orcid":false,"given":"Ning","family":"Yu","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Department of Oral Medicine, Infection, and Immunity Harvard School of Dental Medicine  Boston Massachusetts USA"},{"name":"ADA Forsyth Institute  Cambridge Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7102-9746","authenticated-orcid":false,"given":"William V.","family":"Giannobile","sequence":"additional","affiliation":[{"name":"Division of Periodontology, Department of Oral Medicine, Infection, and Immunity Harvard School of Dental Medicine  Boston Massachusetts 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