{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T18:11:24Z","timestamp":1790273484599,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005089","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7232219"],"award-info":[{"award-number":["7232219"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Orthodontic treatment commonly results in orthodontically induced inflammatory root resorption (OIIRR). This condition arises from excessive orthodontic force, which triggerslocal inflammatory responses and impedes cementoblasts' mineralization capacity. Low\u2010intensity pulsed ultrasound (LIPUS) shows potential in reducing OIIRR. However, the precise mechanisms through which LIPUS reduces OIIRR remain unclear. This study aimed to explore the effects and mechanisms of LIPUS on the mineralization of force\u2010treated cementoblasts and its impact on OIIRR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We established a rat OIIRR model and locally administered LIPUS stimulation for 7 and 14\u2009days. We analyzed root resorption volume, osteoclast differentiation, and the expression of osteocalcin and yes\u2010associated protein 1 (YAP1) using micro\u2010computed tomography (micro\u2010CT), hematoxylin and eosin, tartrate\u2010resistant acid phosphatase, immunofluorescence and immunohistochemistry staining. In\u00a0vitro, we applied compressive force and LIPUS to the immortalized mouse cementoblasts (OCCM30). We assessed mineralization using alkaline phosphatase (ALP) staining, alizarin red staining, real\u2010time quantitative polymerase chain reaction, Western blotting and immunofluorescence staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In rats, LIPUS reduced OIIRR, as evidenced by micro\u2010CT analysis and histological staining. In\u00a0vitro, LIPUS enhanced mineralization of force\u2010treated OCCM30 cells, as indicated by ALP and alizarin red staining, upregulated mRNA expression of mineralization\u2010related genes, and increased protein expression of mineralization markers. Mechanistically, LIPUS activated YAP1 signaling via the cytoskeleton\u2010Lamin A\/C pathway, supported by immunofluorescence and Western blot analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrates that LIPUS promotes mineralization in force\u2010treated cementoblasts and reduces OIIRR by activating YAP1 through the cytoskeletal\u2010Lamin A\/C signaling pathway. These findings provide fresh insights into how LIPUS benefits orthodontic treatment and suggest potential strategies for preventing and treating OIIRR.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13330","type":"journal-article","created":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T05:13:57Z","timestamp":1722662037000},"page":"189-199","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effect of low\u2010intensity pulsed ultrasound on the mineralization of force\u2010treated cementoblasts and orthodontically induced inflammatory root resorption via the Lamin A\/C\u2010Yes associated protein axis"],"prefix":"10.1111","volume":"60","author":[{"given":"Fu","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Orthodontics, Peking University School and Hospital of Stomatology, National Clinical Research Center for Oral Diseases &amp; National Engineering Laboratory for Digital and Material 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