{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T02:47:04Z","timestamp":1788922024462,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,9,29]],"date-time":"2024-09-29T00:00:00Z","timestamp":1727568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007197","name":"U.S. Public Health Service","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007197","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Periodontitis is a prevalent inflammatory disorder affecting the oral cavity, driven by dysbiotic oral biofilm and host immune response interactions. While the major clinical focus of periodontitis treatment is currently controlling oral biofilm, understanding the immune response is crucial to prevent disease progression. Soluble epoxide hydrolase (sEH) inhibition has shown promise in preventing alveolar bone resorption. Triggering receptors expressed on myeloid cells (TREMs) play pivotal roles in regulating inflammation and bone homeostasis, and dysregulation of TREM signaling is implicated in periodontitis. Here, we investigated the impact of sEH inhibition on TREM 1 and 2 expression, associated with inflammatory cytokines, and histologically assessed the inflammatory infiltrate in periodontal tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The experimental periodontitis model was induced by placing a ligature around the upper second molar. For 14\u2009days, animals were treated daily with a sEH inhibitor (TPPU) or vehicle. The alveolar bone loss was examined using a methylene blue stain. Gingival tissues were used to measure the mRNA expression of TREM\u20101, TREM\u20102, IKK\u03b2, NF\u2010\u03baB, IL\u20101\u03b2, IL\u20106, IL\u20108, and TNF\u2010\u03b1 by RT\u2010qPCR. Another set of experiments was performed to determine the histological inflammatory scores.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In a ligature\u2010induced periodontitis model, sEH inhibition prevented alveolar bone loss and reduced TREM1 expression, albeit with a slight elevation compared to the disease\u2010free group. In contrast, TREM2 expression remained elevated, suggesting sustained immunomodulation favoring resolution. The inhibition of sEH reduced the expression of NF\u2010\u03baB, IL\u20101\u03b2, and TNF\u2010\u03b1, while no differences were found in the expression of IL\u20106, IL\u20108, and IKK\u03b2. In histological analysis, sEH inhibition reduced the inflammatory leukocyte infiltrate in periodontal tissues close to the ligature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings underscore the potential of sEH inhibition to modulate periodontal inflammation by regulating TREM\u20101 alongside decreased IL\u20101\u03b2 and TNF\u2010\u03b1 expression, highlighting a promising therapeutic approach for periodontitis management.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13350","type":"journal-article","created":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T02:23:57Z","timestamp":1727663037000},"page":"278-286","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Soluble epoxide hydrolase inhibition impairs triggering receptor expressed on myeloid cells\u20101 in periodontal tissue"],"prefix":"10.1111","volume":"60","author":[{"given":"Breno da Silva","family":"Vargas","sequence":"first","affiliation":[{"name":"Laboratory of Neuroimmune Interface of Pain Research, Faculdade S\u00e3o Leopoldo Mandic Instituto de Pesquisa S\u00e3o Leopoldo Mandic  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno S\u00e9rgio Ferreira","family":"Vargas","sequence":"additional","affiliation":[{"name":"Laboratory of Neuroimmune Interface of Pain Research, Faculdade S\u00e3o Leopoldo Mandic Instituto de Pesquisa S\u00e3o Leopoldo Mandic  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Trindade","family":"Clemente\u2010Napimoga","sequence":"additional","affiliation":[{"name":"Laboratory of Neuroimmune Interface of Pain Research, Faculdade S\u00e3o Leopoldo Mandic Instituto de Pesquisa S\u00e3o Leopoldo Mandic  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce D.","family":"Hammock","sequence":"additional","affiliation":[{"name":"Department of Entomology and UCD Comprehensive Cancer Center University of California  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7517-2830","authenticated-orcid":false,"given":"Henrique B.","family":"Abdalla","sequence":"additional","affiliation":[{"name":"Laboratory of Neuroimmune Interface of Pain Research, Faculdade S\u00e3o Leopoldo Mandic Instituto de Pesquisa S\u00e3o Leopoldo Mandic  Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Van Dyke","sequence":"additional","affiliation":[{"name":"Department of Immunology and Inflammation The ADA Forsyth Institute  Cambridge Massachusetts USA"},{"name":"Department of Oral Medicine, Infection, and Immunity, Faculty of Medicine Harvard University  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo 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