{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T19:29:58Z","timestamp":1789414198780,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study aims to investigate the role of Plexin\u2010B2 in tension\u2010induced osteogenesis of periodontal ligament stem cells (PDLSCs) and its biomechanical mechanism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In vitro, cyclic tension simulated orthodontic forces to assess Plexin\u2010B2 expression in PDLSCs. We then knocked out Plexin\u2010B2 using lentivirus to explore its role in tension\u2010induced osteogenesis. In\u00a0vivo, we used nickel\u2010titanium springs to establish orthodontic tooth movement (OTM) models in mice. Local periodontal Plexin\u2010B2 expression was knocked down using adeno\u2010associated viruses (AAVs) to study its influence on new bone formation under mechanical tension in OTM models. Molecular mechanisms were elucidated by manipulating Plexin\u2010B2 and RhoA expression, assessing related proteins, and observing F\u2010actin and Yes\u2010associated protein (YAP) through immunofluorescence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Plexin\u2010B2 expression in PDLSCs increased under cyclic tension. Decrease of Plexin\u2010B2 reduced the expression of osteogenic protein in PDLSCs and negatively affected new bone formation during OTM. RhoA expression and phosphorylation of ROCK2\/LIMK2\/Cofilin decreased in Plexin\u2010B2 knockout PDLSCs but were reversed by RhoA overexpression. The level of F\u2010actin decreased in Plexin\u2010B2 knockout PDLSCs but increased after RhoA rescue. Nuclear YAP was reduced in Plexin\u2010B2 knockout PDLSCs but increased after RhoA overexpression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Plexin\u2010B2 is involved in tension\u2010induced osteogenesis. Mechanistically, the RhoA signaling pathway, the F\u2010actin arrangement, and the nuclear translocation of YAP are involved in the mechanotransduction of Plexin\u2010B2.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jre.13358","type":"journal-article","created":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T11:18:41Z","timestamp":1730459921000},"page":"1143-1155","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Plexin\u2010\n                    <scp>B2<\/scp>\n                    Mediates Orthodontic Tension\u2010Induced Osteogenesis via the\n                    <scp>RhoA<\/scp>\n                    \/F\u2010Actin\/\n                    <scp>YAP<\/scp>\n                    Pathway"],"prefix":"10.1111","volume":"60","author":[{"given":"Qiming","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoge","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingjian","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinrui","family":"Men","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Stomatology Inner Mongolia Hulunbuir Yakeshi Municipal People's Hospital  Hulunbuir China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7185-0123","authenticated-orcid":false,"given":"Song","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.00824"},{"issue":"11","key":"e_1_2_11_3_1","first-page":"7872","article-title":"Cyclic Tension Promotes 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