{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:13:00Z","timestamp":1787015580560,"version":"build-2736575974"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T00:00:00Z","timestamp":1731974400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA 1206100"],"award-info":[{"award-number":["2022YFA 1206100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82370911"],"award-info":[{"award-number":["82370911"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2025,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n Aims<\/jats:title>\n \n This research sought to assess the efficacy of\n \n Bacillus subtilis<\/jats:italic>\n <\/jats:styled-content>\n (\n \n B. subtilis<\/jats:italic>\n <\/jats:styled-content>\n ) R0179 and explore potential metabolites in mitigating experimental periodontitis in mice induced by\n \n Porphyromonas gingivalis<\/jats:italic>\n <\/jats:styled-content>\n (\n \n P. gingivalis<\/jats:italic>\n <\/jats:styled-content>\n ) ATCC 33277.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n \n B. subtilis<\/jats:italic>\n <\/jats:styled-content>\n R0179 was administered to 8\u2010week\u2010old male C57BL\/6J mice with periodontitis. Oral load of\n \n P. gingivalis<\/jats:italic>\n <\/jats:styled-content>\n ATCC 33277 and periodontal tissue loss were quantified. The cell\u2010free supernatant (CFS) was separated to assess its anti\n \n \u2010\n P. gingivalis<\/jats:styled-content>\n <\/jats:italic>\n effect. Proteomic and metabolomic analyses identified potential antibacterial components in the CFS, further evaluated for anti\u2010\n \n P. gingivalis<\/jats:italic>\n <\/jats:styled-content>\n effects.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n \n B. subtilis<\/jats:italic>\n <\/jats:styled-content>\n R0179 significantly reduced\n \n P. gingivalis<\/jats:italic>\n <\/jats:styled-content>\n ATCC 33277 levels and mitigated periodontal tissue loss in mice. The CFS, rather than inactivated\n \n B. subtilis<\/jats:italic>\n <\/jats:styled-content>\n R0179 cells, exhibited antibacterial activity. Proteomic and metabolomic analyses identified mesaconate and citraconate as key antibacterial agents. Disk diffusion assays confirmed the efficacy of mesaconate against\n \n P. gingivalis<\/jats:italic>\n <\/jats:styled-content>\n , while citraconate had no effect. Mesaconate showed a dose\u2010dependent reduction in\n \n P. gingivalis<\/jats:italic>\n <\/jats:styled-content>\n ATCC 33277 population and periodontal tissue loss in mice.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n These findings highlight\n \n B. subtilis<\/jats:italic>\n <\/jats:styled-content>\n R0179 and its metabolite mesaconate as promising candidates for therapeutic development against periodontitis by inhibiting\n \n P. gingivalis<\/jats:italic>\n <\/jats:styled-content>\n ATCC 33277 effectively.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jre.13363","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T09:19:41Z","timestamp":1732007981000},"page":"617-627","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Mesaconate from\n \n Bacillus subtilis<\/i>\n R0179\n <\/scp>\n Supernatant Attenuates Periodontitis by Inhibiting\n \n Porphyromonas gingivalis<\/i>\n <\/scp>\n in Mice"],"prefix":"10.1111","volume":"60","author":[{"given":"Weiwei","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Preventive Dentistry Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingli","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing People's Republic of 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