{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T23:48:05Z","timestamp":1785368885442,"version":"3.55.0"},"reference-count":53,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T00:00:00Z","timestamp":1732665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82001057"],"award-info":[{"award-number":["82001057"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003787","name":"Natural Science Foundation of Hebei Province","doi-asserted-by":"publisher","award":["H2024112010"],"award-info":[{"award-number":["H2024112010"]}],"id":[{"id":"10.13039\/501100003787","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      To investigate the role of lipopolysaccharide (LPS) from\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      and miR\u2010155\u20105p\u2010enriched exosomes in the formation of foam cells and the occurrence of carotid atherosclerosis (CAS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The CAS tissue samples and plasma from the healthy control group or patients undergoing periodontitis without CAS and with CAS were collected at the Xuanwu Hospital, Capital Medical University. The expression level of miR\u2010155\u20105p was evaluated by immunofluorescent analysis and qRT\u2010PCR. Oil red O staining and lipid accumulation assays were performed to explore the effects of LPS and miR\u2010155\u20105p on mouse macrophage Raw264.7 and human monocytes THP\u20101. The expression levels of lipid\u2010regulated genes were detected by qRT\u2010PCR. Dual\u2010luciferase reporter gene assay and\n                      <jats:italic>DET1<\/jats:italic>\n                      overexpressed or inhibited Raw264.7 cells were used to verify the target gene of exosomal miR\u2010155\u20105p. ApoE\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice were used to confirm the auxo\u2010action of atherosclerosis from exosomal miR\u2010155\u20105p in\u00a0vivo, and LAL assay was used to detect the LPS content.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      miR\u2010155\u20105p was higher in patients with periodontitis and CAS plasma exosomes than those in patients without CAS. The expression of miR\u2010155\u20105p was significantly increased in CAS tissues compared with Normal tissues, and the expression level of miR\u2010155\u20105p was associated with lipid\u2010regulated genes in CAS tissues. MiR\u2010155\u20105p\u2010enriched exosomes accelerated lipid accumulation in macrophage\u2010like cells and promoted the activity of lipid\u2010accumulation genes by targeting\n                      <jats:italic>DET1<\/jats:italic>\n                      . In ApoE\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice, circulating miR\u2010155\u20105p\u2010enriched exosomes captured LPS, and the LPS\u2010laden exosomes conferred plasma access for LPS, triggering the formation of foam cells and the occurrence of CAS.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>miR\u2010155\u20105p enriched exosomes capture and escort LPS to the atherosclerotic sites, licensing the formation of foam cells and thus promoting CAS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jre.13369","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T02:05:25Z","timestamp":1732759525000},"page":"1132-1142","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Exosomal\n                    <scp>miR<\/scp>\n                    \u2010155\u20105p Facilitates Lipopolysaccharide Transport and Foam Cell Formation: A Novel Link Between Periodontitis and Atherosclerosis"],"prefix":"10.1111","volume":"60","author":[{"given":"Wen\u2010Wen","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Stomatology, Xuanwu Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing\u2010Xiang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Peking University School and Hospital of Stomatology  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, Xuanwu Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Ran","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Xuanwu Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian\u2010Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Xuanwu Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Xuanwu Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Xuanwu Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5379-9543","authenticated-orcid":false,"given":"Ying","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Xuanwu Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Xuanwu Hospital Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,27]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.123.318974"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph20186721"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2023.107255"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11121979"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.120.316770"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-1924-7_1"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2204360218"},{"key":"e_1_2_13_9_1","article-title":"Chronic Periodontitis is Associated With Cerebral Atherosclerosis\u2014A Nationwide Study","volume":"12","author":"Patel U. 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