{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:14:10Z","timestamp":1787015650081,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T00:00:00Z","timestamp":1736726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>The clinical outcomes of a variety of surgical procedures highly depend on tissue repair and show high variability among patients. There is a gap in the literature on how the host inflammatory response, the microbiome, and the interplay between them can influence oral mucosa healing. In this pilot study, we aimed to evaluate the microbiome and biomarkers profiles in patients who had desired versus undesired wound healing in the palatal mucosa.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Seventeen patients underwent a free gingival graft (FGG) for socket preservation. Palatal wound closure (WC) and epithelization (EPT) were assessed clinically. Biofilm from the palatal wound was collected before the surgical procedure and 3, 7, 14, and 30\u2009days postoperatively. The inflammatory exudate was sampled on Days 3 and 7. At 14\u2009days posttreatment, patients were classified into two groups based on EPT rates: (1) undesired healing (UH) and (2) desired healing (DH).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      No difference was observed in alfa diversity over time or between groups. In beta diversity, both UH and DH showed microbiome changes on Days 3\u20137 and 7, respectively, compared with the baseline (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.01), returning to its initial condition 30\u2009days later. There was a trend toward a different microbiome profile between groups on Day 7 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.08). Bacterium composition in DH showed a balance between healthy species and oral pathogens over time, whereas UH composition was characterized by microorganisms correlated with epithelium invasion\/cytotoxicity; virulence factor upregulation; and oral diseases, such as periodontitis and aphthous stomatitis, until Day 30. UH showed an increase in IL\u20106, MCP\u20101, and MIP\u20101\u03b1 over time, and DH showed a decrease in TIMP\u20101, IL\u20101\u03b2, and MIP\u20101\u03b1. On Days 3 and 7, MIP\u20101\u03b1 and MMP\u20102 showed greater concentrations of DH in the intergroup assessment, and MCP\u20101 increased on Day 7 in UH.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Specific microbiome\/inflammatory profiles are associated with DH and UH.<\/jats:p>\n                    <jats:p>\n                      <jats:bold>Trial Registration:<\/jats:bold>\n                      NCT05171400\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jre.13373","type":"journal-article","created":{"date-parts":[[2025,1,13]],"date-time":"2025-01-13T05:05:38Z","timestamp":1736744738000},"page":"664-675","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Microbiome and Inflammatory Biomarkers Associated With Palatal Wound Healing"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9468-0729","authenticated-orcid":false,"given":"Mauro Pedrine","family":"Santamaria","sequence":"first","affiliation":[{"name":"College of Dentistry University of Kentucky  Lexington Kentucky USA"},{"name":"Division of Periodontics, Institute of Science and Technology, S\u00e3o Jos\u00e9 dos Campos S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2518-0135","authenticated-orcid":false,"given":"Ingrid Fernandes","family":"Mathias\u2010Santamaria","sequence":"additional","affiliation":[{"name":"College of Dentistry University of Kentucky  Lexington Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5685-0002","authenticated-orcid":false,"given":"Ana Carolina","family":"Ferreira Bonaf\u00e9","sequence":"additional","affiliation":[{"name":"Division of Periodontics, Institute of Science and Technology, S\u00e3o Jos\u00e9 dos Campos S\u00e3o Paulo State University (UNESP)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8885-8604","authenticated-orcid":false,"given":"Octavio A.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"College of Dentistry University of Kentucky  Lexington Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4473-1126","authenticated-orcid":false,"given":"Sreenatha","family":"Kirakodu","sequence":"additional","affiliation":[{"name":"College of Dentistry University of Kentucky  Lexington Kentucky USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9333-4349","authenticated-orcid":false,"given":"Mabelle de 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