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Real\u2010time quantitative PCR (RT\u2010qPCR) and Western blotting were used to assess the expression of genes and proteins related to apoptosis, oxidative stress, and mitochondrial dysfunction. Fluorescence staining was employed to detect changes in cellular ROS (reactive oxygen species), MMP (mitochondrial membrane potential), and mtROS (mitochondrial ROS) expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that miR\u2010223\u20103p targeted FoxO3 to regulate apoptosis in cementoblasts under hypoxic conditions. Moreover, hypoxia\u2010induced FoxO3 increased oxidative stress and induced mitochondrial dysfunction in cementoblasts, resulting in cell apoptosis. Administration of the ROS inhibitor NAC (N\u2010acetyl cysteine) effectively reversed FoxO3\u2010induced oxidative stress and mitochondrial dysfunction, thereby rescuing cell apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>miR\u2010223\u20103p targets FoxO3 and regulates the apoptosis of cementoblasts by improving oxidative stress and mitochondrial dysfunction. These findings may offer new insights into the mechanism of OIIRR.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13384","type":"journal-article","created":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T07:55:27Z","timestamp":1745222127000},"page":"923-936","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>miR<\/scp>\u2010223\u20103p Mitigates Mitochondrial Dysfunction and Cementoblast Apoptosis in Orthodontic Root Resorption via <scp>FoxO3<\/scp>"],"prefix":"10.1111","volume":"60","author":[{"given":"Wei","family":"He","sequence":"first","affiliation":[{"name":"College of Stomatology Chongqing Medical University  Chongqing China"},{"name":"Chongqing Key Laboratory of Oral Diseases  Chongqing China"},{"name":"Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education  Chongqing 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