{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:01:18Z","timestamp":1789624878854,"version":"build-2803163510"},"reference-count":53,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T00:00:00Z","timestamp":1739750400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To study the differential presence of amyloid\u2010\u03b2 and bacterial lipopolysaccharide (LPS) in freshly extracted titanium implants, either affected by peri\u2010implantitis (PI) or explanted by other causes, and to address a method for removal LPS and amyloid\u2010\u03b2 from contaminated surfaces.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010four explanted implants were harvested from patients with (<jats:italic>n<\/jats:italic>\u2009=\u200912) or without (<jats:italic>n<\/jats:italic>\u2009=\u200912) peri\u2010implantitis, and their surfaces were analyzed by attenuated total reflectance (ATR) and Fourier transform infrared spectroscopy (FTIR) to localize amyloid\u2010\u03b2 and LPS. Presence of amyloid\u2010\u03b2 on the implants surfaces was further analyzed by light microscopy after specific amyloid staining with Congo red. Titanium discs were contaminated with LPS and amyloid\u2010\u03b2, these discs as well as six contaminated implants were treated with 0.25% NaOCl to assess its decontamination ability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>LPS and amyloid\u2010\u03b2 were observed at PI affected implant surfaces, but not in implants extracted by other causes. 0.25% NaOCl application was an efficient method for removing LPS and amyloid\u2010\u03b2 from titanium surfaces.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The concurrent presence of LPS and amyloid\u2010\u03b2 on the surface of implants affected by PI was demonstrated and it may act as potential comediators of PI inflammatory process. Eliminating these products from implants surfaces is possible after a proteolytic agent (0.25% NaOCl) application.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13386","type":"journal-article","created":{"date-parts":[[2025,2,17]],"date-time":"2025-02-17T08:23:47Z","timestamp":1739780627000},"page":"813-822","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Amyloid\u2010\u03b2 and Bacterial Lipopolysaccharide at Implants With Peri\u2010Implantitis: Ex\u00a0Vivo Colocalization and Decontamination Protocol"],"prefix":"10.1111","volume":"60","author":[{"given":"Manuel","family":"Toledano\u2010Osorio","sequence":"first","affiliation":[{"name":"Postgraduate Program of Specialization in Periodontology, Faculty of Dentistry University Complutense of Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6553-931X","authenticated-orcid":false,"given":"Carolina","family":"Cifuentes\u2010Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Toledano","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry University of Granada  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6293-5755","authenticated-orcid":false,"given":"Mariano","family":"Sanz","sequence":"additional","affiliation":[{"name":"ETEP (Etiology and Therapy of Periodontal and Peri\u2010Implant Diseases) Research Group. 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