{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T19:47:57Z","timestamp":1787600877313,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the role of Toll\u2010like receptor 7 (TLR7) in periodontitis and its potential mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>TLR7 expression in periodontal tissues was analyzed using GEO database single\u2010cell sequencing data and confirmed with gingival samples from healthy individuals and periodontitis patients. In\u00a0vivo, a mouse model of periodontitis was used to assess the effect of M5049, the TLR7's inhibitor, on alveolar bone loss and inflammation. In\u00a0vitro, mouse bone marrow\u2010derived macrophages (BMDMs) were treated with Imiquimod to activate TLR7. The inflammatory response was further evaluated using <jats:italic>Pg<\/jats:italic>\u2010LPS\u2010stimulated BMDMs, with TLR7 knockdown and NF\u2010\u03baB inhibition by PDTC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TLR7 expression in the gingival tissues of periodontitis patients was significantly elevated compared to healthy gingival tissues. In\u00a0vivo, TLR7 inhibition reduced bone loss and inflammation, with decreased osteoclast formation and cytokine expression. In\u00a0vitro, activation of TLR7 heightened inflammation; conversely, TLR7 knockdown and NF\u2010\u03baB inhibition diminished cytokine expression, suggesting a role for NF\u2010\u03baB in TLR7\u2010mediated inflammatory responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TLR7 is upregulated in periodontitis and may promote the progression of the disease by activating the NF\u2010\u03baB signaling pathway, potentially serving as a therapeutic target. The findings reveal a novel role for TLR7 in periodontitis and highlight the TLR7\u2010NF\u2010\u03baB axis as a key pathway in disease pathogenesis, with broader implications for understanding and treating inflammatory conditions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13394","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T09:06:40Z","timestamp":1741684000000},"page":"823-834","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Toll\u2010Like Receptor 7 Promotes Periodontal Inflammation and Alveolar Bone Resorption Through the <scp>NF<\/scp>\u2010<scp>\u03baB<\/scp> Signaling Pathway"],"prefix":"10.1111","volume":"60","author":[{"given":"Rui","family":"Jing","sequence":"first","affiliation":[{"name":"Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Tongji Research Institute of Stomatology, Department of Periodontology, Stomatological Hospital and 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