{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T17:00:45Z","timestamp":1783443645874,"version":"3.54.6"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Periodontal diseases pose significant challenges to oral health, making the regeneration of periodontal tissues a critical therapeutic goal. The goal is to restore dental function by repairing damaged tissue and reconstructing the healthy connective structure between the teeth and the alveolar bone. This study aimed to investigate the effects of selcopintide (SCPT) on the differentiation of periodontal ligament cells (PDLCs), cementoblasts, and osteoblasts in\u00a0vitro, as well as the regeneration of periodontal tissue using a periodontal tissue defect model in dogs in\u00a0vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression of periodontal tissue marker genes, including periostin (POSTN), cementum attachment protein (CAP), dentin matrix protein 1 (DMP1), and bone sialoprotein (BSP), was investigated in\u00a0vitro. Chronic one\u2010wall intrabony defects were created in a total of 12 beagle dogs (<jats:italic>n<\/jats:italic>\u2009=\u20096 at 6 and 12\u2009weeks, respectively), and the surgical sites were treated with no treatment, guided tissue regeneration (GTR), GTR with SCPT 50\u2009\u03bcg\/0.1\u2009mL, 100\u2009\u03bcg\/0.1\u2009mL, and 250\u2009\u03bcg\/0.1\u2009mL. The effects of SCPT on the regeneration of periodontal tissues, such as periodontal ligament (PDL), cementum, and bone, were analyzed in\u00a0vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SCPT influenced the proliferation and differentiation of cementoblasts and PDLCs. Real\u2010time polymerase chain reaction analysis showed that SCPT upregulated the expression of POSTN, CAP, DMP1, and BSP compared to the control. In the periodontal defect model, SCPT regenerated the periodontal complex. Additionally, the arrangement of the newly formed PDL\u2010like fibers was perpendicular to the newly formed cementum and alveolar bone, similar to Sharpey's fibers in natural teeth, compared with the control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this preclinical study, histological and immunohistochemical analyses suggest that GTR with SCPT might be associated with increased periodontal ligament attachment and enhanced cementum and alveolar bone formation. Additional research with a larger sample size is needed to establish the optimal therapeutic protocols and validate the regenerative potential of SCPT.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jre.13395","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T07:40:22Z","timestamp":1742370022000},"page":"835-846","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of Selcopintide on Periodontal Tissue Regeneration in Chronic One\u2010Wall Intrabony Defect Model"],"prefix":"10.1111","volume":"60","author":[{"given":"Young\u2010Chang","family":"Ko","sequence":"first","affiliation":[{"name":"Department of Periodontology, School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong\u2010Seol","family":"Lee","sequence":"additional","affiliation":[{"name":"Laboratory for the Study of Regenerative Dental Medicine, Department of Oral Histology and Developmental Biology, School of Dentistry Seoul National University  Seoul Republic of Korea"},{"name":"Regenerative Dental Medicine R and D Center HysensBio Co. Ltd  Gwacheon\u2010si Gyeonggi\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang\u2010Jo","family":"Seol","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Moo","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9809-2630","authenticated-orcid":false,"given":"Ki\u2010Tae","family":"Koo","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joo\u2010Cheol","family":"Park","sequence":"additional","affiliation":[{"name":"Laboratory for the Study of Regenerative Dental Medicine, Department of Oral Histology and Developmental Biology, School of Dentistry Seoul National University  Seoul Republic of Korea"},{"name":"Regenerative Dental Medicine R and D Center HysensBio Co. Ltd  Gwacheon\u2010si Gyeonggi\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won\u2010Jun","family":"Shon","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry, School of Dentistry and Dental Research Institute Seoul National University School of Dentistry  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5508-442X","authenticated-orcid":false,"given":"Jungwon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dentistry and Dental Research Institute Seoul National University  Seoul Republic of Korea"},{"name":"One\u2010Stop Specialty Center Seoul National University Dental Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,19]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12732"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13769"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12344"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12226"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12477"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2203660183"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.961898"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma13204618"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13621"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000410"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01566.x"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2004.75.2.229"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2011.41.6.285"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.23-0617"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.22-0586"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1994.tb00302.x"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2022.105531"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina60050773"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2300800040"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2303840192"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1151322"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.3241"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano12213878"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.01369.x"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13381"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reth.2023.01.002"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.070624"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23499"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2006.00157.x"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.13395","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T06:55:31Z","timestamp":1760338531000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.13395"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,19]]},"references-count":29,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1111\/jre.13395"],"URL":"https:\/\/doi.org\/10.1111\/jre.13395","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,19]]},"assertion":[{"value":"2024-12-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}