{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T19:50:47Z","timestamp":1792525847458,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:00:00Z","timestamp":1747353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:00:00Z","timestamp":1747353600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      Periodontitis, a chronic inflammatory disease, is associated with accelerated cellular senescence. This study aims to explore how\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      (\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. gingivalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      ) outer membrane vesicles (OMVs) induce senescence and senescence\u2010associated secretory phenotype (SASP) in human gingival fibroblasts (HGFs), focusing on the role of lncRNAs in promoting periodontitis progression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In vivo, gingival senescence was assessed via immunohistochemistry while bone loss was evaluated using micro\u2010CT, HE and TRAP staining. In\u00a0vitro, HGFs senescence was validated using CCK\u20108, SA\u2010\u03b2\u2010gal staining, western blot and immunofluorescence assays. IL21R\u2010AS1 was transfected with targeted siRNAs and overexpression plasmids to verify its function. Dual\u2010luciferase reporter assays and rescue experiments were conducted to elucidate the competing endogenous RNA (ceRNA) mechanism of IL21R\u2010AS1. Upstream transcription factors of IL21R\u2010AS1 were validated by CUT&amp;Tag\u2010qPCR and western blot.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. gingivalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      OMVs induced gingival tissue senescence and alveolar bone loss in\u00a0vivo. In\u00a0vitro,\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. gingivalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      OMVs promoted HGFs senescence and SASP via IL21R\u2010AS1. IL21R\u2010AS1 acted as a ceRNA by sponging miR\u2010500a\u20103p, thereby enhancing the regulation of FBXW7. Moreover,\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. gingivalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      OMVs activated TLR4 and increased PAX5 expression, leading to elevated IL21R\u2010AS1 levels.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. gingivalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      OMVs promote HGFs senescence SASP by the upregulation of IL21R\u2010AS1, which acts as a ceRNA, mediating the miR\u2010500a\u20103p\/FBXW7 axis and contributes to the progression of periodontitis. These findings offer novel insights into the molecular mechanisms of OMVs\u2010induced senescence and periodontitis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jre.13419","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T05:49:52Z","timestamp":1747374592000},"page":"203-215","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>Porphyromonas gingivalis<\/i>\n                    Outer Membrane Vesicles Promote Gingival Fibroblasts Senescence via\n                    <scp>IL21R<\/scp>\n                    \u2010\n                    <scp>AS1<\/scp>"],"prefix":"10.1111","volume":"61","author":[{"given":"Zirui","family":"Li","sequence":"first","affiliation":[{"name":"Department of Periodontology The Affiliated Stomatological Hospital of Nanjing Medical University  Nanjing China"},{"name":"State Key Laboratory Cultivation Base of Research, 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