{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T05:43:56Z","timestamp":1783575836704,"version":"3.55.0"},"reference-count":69,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DE027980"],"award-info":[{"award-number":["R01DE027980"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DE021052"],"award-info":[{"award-number":["R01DE021052"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Elevated levels of Herpes Simplex Virus 1 (HSV\u20101) have been reported in periodontitis, however, the tropism and relationship with periodontal inflammation are poorly characterized. This study investigated how inflammation affects viral tropism toward human periodontal ligament stem cells (hPDLSCs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>HSV\u20101 gB and gD transcripts in healthy and diseased human gingiva were measured by RT\u2010qPCR and confirmed in HSV\u20101\u2010infected murine gingiva. HSV\u20101 infection\u00a0in hPDLSCs was analyzed by imaging and flow cytometry. hPDLSCs were individually treated with IL\u20106, TNF\u2010\u03b1, GMCSF, IL\u201010, or PgLPS, and HSV\u20101 replication was assessed by infecting with the 17 GFP strain. Lineage markers in virally infected hPDLSCs during osteogenic differentiation were measured by RT\u2010qPCR and immunofluorescence in\u00a0vitro and validated in\u00a0vivo. Mice subjected to ligature\u2010induced periodontitis (LIP) and infected with HSV\u20101 were examined for gingival histology, inflammatory cytokines, and alveolar bone loss.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Inflamed human gingiva showed higher expression of viral transcripts compared to healthy controls. In mouse oral HSV\u20101 infection, gB and gD expression increased over time, with higher levels in mice with ligature\u2010induced periodontitis. Virus infected hPDLSCs challenged with inflammatory mediators or PgLPS showed higher GFP, while IL\u201010 treatment attenuated GFP levels. Importantly, HSV\u20101 17 GFP infection affected osteoblast lineage commitment by promoting the expression of key transcription factors in\u00a0vitro and in\u00a0vivo. Compared to the LIP alone group, higher levels of inflammatory markers and bone loss were evident in HSV\u20101 infected with LIP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>hPDLSCs are trophic to HSV\u20101 in\u00a0vitro and in\u00a0vivo, with periodontal inflammation playing a significant role in viral tropism.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jre.70022","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T11:44:01Z","timestamp":1753789441000},"page":"1265-1279","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Herpesvirus Simplex Virus\u20101 Exploits Inflammation to Infect Periodontal Stem Cells and Disrupt Lineage Commitment"],"prefix":"10.1111","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6044-0209","authenticated-orcid":false,"given":"Araceli","family":"Valverde","sequence":"first","affiliation":[{"name":"Department of Periodontics, College of Dentistry University of Illinois Chicago  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0187-2081","authenticated-orcid":false,"given":"Raza Ali","family":"Naqvi","sequence":"additional","affiliation":[{"name":"Department of Periodontics, College of Dentistry University of Illinois Chicago  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8991-2377","authenticated-orcid":false,"given":"Yinghua","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Oral Biology University of Illinois Chicago  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1542-6202","authenticated-orcid":false,"given":"Alireza","family":"Moshaverinia","sequence":"additional","affiliation":[{"name":"Division of Advanced Prosthodontics, School of Dentistry University of California  Los Angeles California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"George","sequence":"additional","affiliation":[{"name":"Department of Oral Biology University of Illinois Chicago  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3039-6953","authenticated-orcid":false,"given":"Deepak","family":"Shukla","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology University of Illinois Chicago  Chicago Illinois USA"},{"name":"Department of Ophthalmology University of Illinois Chicago  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"Martinez","sequence":"additional","affiliation":[{"name":"Posgrado de Periodoncia, Facultad de Odontologia Universidad Autonoma de Nuevo Le\u00f3n  Monterrey Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"Chapa","sequence":"additional","affiliation":[{"name":"Posgrado de Periodoncia, Facultad de Odontologia Universidad Autonoma de Nuevo Le\u00f3n  Monterrey Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvador","family":"Nares","sequence":"additional","affiliation":[{"name":"Department of Periodontics, College of Dentistry University of Illinois Chicago  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7436-3056","authenticated-orcid":false,"given":"Afsar R.","family":"Naqvi","sequence":"additional","affiliation":[{"name":"Department of Periodontics, College of Dentistry University of Illinois Chicago  Chicago Illinois USA"},{"name":"Department of Microbiology and Immunology University of Illinois Chicago  Chicago Illinois USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2005.03.003"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2011.00413.x"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12344"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.38"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12297"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00141.x"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)31146-8"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3402\/jom.v5i0.22766"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01865-15"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2008.00279.x"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.03207"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2311"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.5726"},{"issue":"3","key":"e_1_2_12_15_1","first-page":"2199","article-title":"The Treatment of Systematically Transplanted Gingival Mesenchymal Stem Cells in Periodontitis in Mice","volume":"17","author":"Sun W.","year":"2019","journal-title":"Experimental and Therapeutic Medicine"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-17-1961"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.8.4074-4084.2004"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2008.11.018"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13040"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12404"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0902-0055.2002.00118.x"},{"issue":"11","key":"e_1_2_12_22_1","article-title":"Cytomegalovirus Infection in Symptomatic Periapical Pathosis","volume":"38","author":"Slots J.","year":"2004","journal-title":"International Endodontic Journal"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2016.10.006"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2010.09.009"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1834-7819.2011.01360.x"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-014-1250-1"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2018.03.001"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dib.2018.05.020"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02099"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00433"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2081"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbior.2021.100829"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9010072"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12393"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241511989"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2018.10.004"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-022-02716-3"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509340867"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12331"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/idj.12630"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.2144\/fsoa-2023-0062"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-7757-2021-0423"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/iid3.70044"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12298"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.13715"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.66.2.61"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-481"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.617578"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.240309797"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1177\/15353702231187645"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19020360"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40496-013-0010-7"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/4709572"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jobcr.2024.07.003"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01175"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008207.2014.923850"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-023-03416-x"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0043-1761423"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens13010058"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.3390\/v15010225"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2007.06.011"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009847"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.29684"},{"key":"e_1_2_12_65_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-1807"},{"key":"e_1_2_12_66_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200113799"},{"key":"e_1_2_12_67_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02109-15"},{"key":"e_1_2_12_68_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00202-10"},{"key":"e_1_2_12_69_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00621-21"},{"issue":"5","key":"e_1_2_12_70_1","first-page":"7526","article-title":"MicroRNA\u2010H4\u20105p Encoded by HSV\u20101 Latency\u2010Associated Transcript Promotes Cell Proliferation, Invasion and Cell Cycle Progression via p16\u2010Mediated PI3K\u2010Akt Signaling Pathway in SHSY5Y Cells","volume":"8","author":"Zhao H.","year":"2015","journal-title":"International Journal of Clinical and Experimental Medicine"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.70022","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jre.70022","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jre.70022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T07:33:11Z","timestamp":1770881591000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jre.70022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,29]]},"references-count":69,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/jre.70022"],"URL":"https:\/\/doi.org\/10.1111\/jre.70022","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,29]]},"assertion":[{"value":"2025-04-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}