{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:58:12Z","timestamp":1792205892952,"version":"4.4.0"},"reference-count":60,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T00:00:00Z","timestamp":1758067200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>This study aims to investigate the effect of inflammation on the senescence phenotype and osteogenic capacity of aged periodontal ligament cells (PDLCs), and to explore the regulatory role of the NF\u2010\u03baB signalling pathway in the osteogenesis of aged PDLCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Human PDLCs were isolated, and two ageing models were used: replicative senescence and etoposide treatment. The proliferation and migration of PDLCs were tested with the cell counting kit\u20108 assay, 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine staining, and scratch test. Proinflammatory cytokine levels were tested using enzyme\u2010linked immunosorbent assay and real time\u2013quantitative polymerase chain reaction. Osteogenic differentiation was evaluated through alkaline phosphatase activity, Alizarin Red S staining, and calcium quantification. Expression levels of nuclear factor kappa\u2010B (NF\u2010\u03baB) and c\u2010JUN pathway\u2010related proteins were analyzed through Western blotting.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Inflammatory stimulation enhanced the senescence phenotype in both young and aged PDLCs and inhibited osteogenic differentiation in aged PDLCs. During cellular ageing, NF\u2010\u03baB signalling downregulated the osteogenic differentiation of PDLCs by suppressing forkhead box O3a (FOXO3a) and c\u2010JUN. Conversely, under exogenous inflammatory stimulation, NF\u2010\u03baB signalling inhibited osteogenesis by promoting FOXO3a phosphorylation and increasing c\u2010JUN expression, with p21 exerting a synergistic inhibitory effect on osteogenic differentiation in aged PDLCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Inflammation aggravates cellular senescence and suppresses osteogenic differentiation in aged PDLCs through the NF\u2010\u03baB\/FOXO3a\/c\u2010JUN signalling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jre.70037","type":"journal-article","created":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T10:14:52Z","timestamp":1758104092000},"page":"292-308","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Inflammation Affects the Osteogenic Differentiation of Aged Periodontal Ligament Cells via\n                    <scp>NF<\/scp>\n                    \u2010\n                    <scp>\u03baB<\/scp>\n                    \/\n                    <scp>FOXO3a<\/scp>\n                    \/c\u2010\n                    <scp>JUN<\/scp>\n                    Signalling"],"prefix":"10.1111","volume":"61","author":[{"given":"Luying","family":"Zhu","sequence":"first","affiliation":[{"name":"Faculty of Dentistry The University of Hong Kong  Hong Kong China"},{"name":"Stomatological Hospital, School of Stomatology Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyuan","family":"Tang","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry The University of Hong Kong  Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renjie","family":"Hu","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry The University of Hong Kong  Hong Kong 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