{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T14:02:58Z","timestamp":1784296978792,"version":"3.55.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>This study evaluated the potential of a beta\u2010defensin\u20103 mimetic peptide (BDMP), a synthetic cell\u2010penetrating peptide with antimicrobial and immunomodulatory properties, as an adjunctive therapeutic approach for periodontitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>BDMP was formulated in a hydroxyethyl cellulose (HEC) gel and assessed for binding affinity, release kinetics, and ability to penetrate cells and gingival tissues. Anti\u2010inflammatory and osteoclast\u2010related signaling pathways were examined in\u00a0vitro using RAW264.7 macrophages stimulated with lipopolysaccharide (LPS). Effects on osteogenic recovery were evaluated in periodontal ligament stem cells (PDLSCs) under inflammatory conditions. Antimicrobial activity against multispecies biofilms was analyzed by confocal microscopy. In a ligature\u2010induced experimental periodontitis model in beagle dogs, BDMP gel was compared with a subgingival instrumentation (SI)\u2010only (standard\u2010of\u2010care) control, and minocycline gel was included as an active adjunctive comparator. Clinical parameters, inflammatory markers, microbial load, radiographs, micro\u2010CT images, and histology were evaluated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In vitro, BDMP reduced histone deacetylase 5 (HDAC5) phosphorylation and attenuated downstream NF\u2010\u03baB\u2013associated inflammatory signaling without altering upstream kinase activity. BDMP decreased osteoclast differentiation, reduced inflammatory cytokine transcription, and partially restored osteogenic capacity in LPS\u2010stimulated PDLSCs. BDMP also demonstrated broad\u2010spectrum antimicrobial activity and disrupted mature multispecies biofilms. In\u00a0vivo, BDMP resulted in greater reductions in gingival inflammation, bleeding, IL\u20101\u03b2 levels, and oral spirochetes over 12\u2009weeks compared with the SI\u2010only control. Radiographic images provided qualitative support for reduced bone loss, which was corroborated by micro\u2010CT and histology, indicating attenuation of alveolar bone resorption. When compared with the combination of SI and minocycline arm, BDMP showed comparable or greater improvements in several inflammatory and microbiological parameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>BDMP exhibited sustained antimicrobial and anti\u2010inflammatory activity and attenuated bone loss in a beagle periodontitis model when used alongside standard SI therapy. These findings support BDMP as a promising adjunctive therapeutic candidate for managing periodontal inflammation and biofilm\u2010associated disease, although further studies are needed to confirm long\u2010term safety and to define its mechanistic contributions to periodontal tissue preservation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jre.70079","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T10:34:48Z","timestamp":1770114888000},"page":"586-605","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Multifunctional \u03b2\u2010Defensin\u20103 Mimetic Peptide Modulates Host\u2013Biofilm Interactions and Reduces Bone Loss in Periodontitis"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-3495-2189","authenticated-orcid":false,"given":"Beom Soo","family":"Jo","sequence":"first","affiliation":[{"name":"Central Research Institute Nano Intelligent Biomedical Engineering Corporation (NIBEC)  Seoul Korea"},{"name":"Department of Dental Regenerative Biotechnology and Dental Research Institute, School of Dentistry Seoul National University  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1143-3115","authenticated-orcid":false,"given":"Dong Woo","family":"Lee","sequence":"additional","affiliation":[{"name":"Central Research Institute Nano Intelligent Biomedical Engineering Corporation (NIBEC)  Seoul Korea"},{"name":"Department of Dental Regenerative Biotechnology and Dental Research Institute, School of Dentistry Seoul National University  Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Dental Regenerative Biotechnology and Dental Research Institute, School of Dentistry Seoul National University  Seoul 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