{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:13:49Z","timestamp":1762298029503},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,8,5]],"date-time":"2013-08-05T00:00:00Z","timestamp":1375660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Small Animal Practice"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p><jats:bold>To evaluate faecal and clinical scores and presence of several enteropathogens possibly implicated in the development of diarrhoea in pups aged between 6 and 16 weeks independently of their health status.<\/jats:bold><\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:bold>Pups were selected from pet shops and breeding facilities and assigned a faecal and clinical score. Standard isolation methods were used to determine presence of parasites, viruses and bacteria in faecal samples. For<jats:italic>Escherichia coli<\/jats:italic>, virulence genes were assessed by multiplex polymerase chain reaction.<\/jats:bold><\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:bold>Fifty\u2010six pups were included in this study. Eighteen had no diarrhoea, 22 had no significant clinical signs related to gastroenteritis. Samples were positive for<jats:italic>Toxocara canis<\/jats:italic>(n=29),<jats:italic>Giardia duodenalis<\/jats:italic>(n=35),<jats:italic>Cystoisospora<\/jats:italic>(n=22),<jats:italic>E. coli<\/jats:italic>(n=47) and<jats:italic>Clostridium perfringens<\/jats:italic>(n=20). In four<jats:italic>E. coli<\/jats:italic>positive samples, genes were detected that correlate with pathogenicity in other animal species. A significant positive correlation between the presence of<jats:italic>T. canis<\/jats:italic>and faecal score was found.<\/jats:bold><\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical Significance<\/jats:title><jats:p><jats:bold>Puppies obtained from a pet shop or breeding facility have a high risk of gastrointestinal disease. Furthermore, infectious agents may be present independently of faecal or clinical score. The identification of possible pathogenic<jats:italic>E. coli<\/jats:italic>strains suggests that their role in diarrhoea warrant further investigation.<\/jats:bold><\/jats:p><\/jats:sec>","DOI":"10.1111\/jsap.12119","type":"journal-article","created":{"date-parts":[[2013,8,5]],"date-time":"2013-08-05T13:24:11Z","timestamp":1375709051000},"page":"475-480","source":"Crossref","is-referenced-by-count":15,"title":["Enteropathogens in pups from pet shops and breeding facilities"],"prefix":"10.1111","volume":"54","author":[{"given":"S.","family":"Dupont","sequence":"first","affiliation":[{"name":"Department of Medicine and Clinical Biology of Small Animals, Faculty of Veterinary Medicine Ghent University Salisburylaan 133 Merelbeke B\u20109820 Belgium"},{"name":"Veos N.V. 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