{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T21:13:08Z","timestamp":1768770788228,"version":"3.49.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,1,22]],"date-time":"2018-01-22T00:00:00Z","timestamp":1516579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Small Animal Practice"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To investigate evidence for selected vector\u2010borne pathogen infections in dogs with pericardial effusion living in a Mediterranean area in which several canine vector\u2010borne diseases are endemic.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Archived EDTA blood (n=68) and pericardial fluid samples (n=58) from dogs with pericardial effusion (n=68) were included. Dogs without pericardial effusion examined for other reasons were included as controls (n=60). Pericardial effusion was classified as neoplastic in 40 dogs, idiopathic in 23 dogs and of unknown aetiology in 5 dogs. Real\u2010time PCR was performed for <jats:italic>Leishmania infantum<\/jats:italic>, <jats:italic>Ehrlichia\/Anaplasma<\/jats:italic> species, <jats:italic>Hepatozoon canis<\/jats:italic>, <jats:italic>Babesia<\/jats:italic> species, <jats:italic>Rickettsia<\/jats:italic> species and <jats:italic>Bartonella<\/jats:italic> species, and sequencing of PCR products from positive samples was used to confirm species specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Vector\u2010borne pathogens were found in 18 dogs: 16 of 68 dogs with pericardial effusion (23\u00b75%) and two of 60 control dogs (3\u00b73%). Positive dogs demonstrated DNA of <jats:italic>Leishmania infantum<\/jats:italic> (n=7), <jats:italic>Anaplasma platys<\/jats:italic> (n=2, one dog coinfected with <jats:italic>Leishmania infantum<\/jats:italic>), <jats:italic>Babesia canis<\/jats:italic> (n=5), <jats:italic>Babesia gibsoni<\/jats:italic> (n=3) and <jats:italic>Hepatozoon canis<\/jats:italic> (n=2). Vector\u2010borne pathogens were more commonly detected among dogs with pericardial effusion than controls (P=0\u00b7001). There was no relationship between aetiology of the pericardial effusion and evidence of vector\u2010borne pathogens (P=0\u00b7932).<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical Significance<\/jats:title><jats:p>Vector\u2010borne pathogens are often detected in dogs with pericardial effusion and require further investigation, especially in dogs with idiopathic pericardial effusion. PCR can provide additional information about the potential role of vector\u2010borne pathogens in dogs with pericardial effusion living in endemic areas.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jsap.12817","type":"journal-article","created":{"date-parts":[[2018,1,22]],"date-time":"2018-01-22T15:15:27Z","timestamp":1516634127000},"page":"248-252","source":"Crossref","is-referenced-by-count":13,"title":["PCR evaluation of selected vector\u2010borne pathogens in dogs with pericardial effusion"],"prefix":"10.1111","volume":"59","author":[{"given":"M.\u2010D.","family":"Tabar","sequence":"first","affiliation":[{"name":"Hospital Veterinario San Vicente  Alicante 03540 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Movilla","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednic Veterinari Universitat Aut\u00f2noma de Barcelona  Barcelona 08193 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Serrano","sequence":"additional","affiliation":[{"name":"Vetgenomics Parc de recerca UAB, Edifici Eureka  Barcelona 08193 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Altet","sequence":"additional","affiliation":[{"name":"Vetgenomics Parc de recerca UAB, Edifici Eureka  Barcelona 08193 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Francino","sequence":"additional","affiliation":[{"name":"Servei Veterinari de Gen\u00e8tica Molecular Facultad de Veterinaria, Universitat Aut\u00f2noma de Barcelona  08193 Bellaterra Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Roura","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednic Veterinari Universitat Aut\u00f2noma de Barcelona  Barcelona 08193 Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,1,22]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1532-950X.1995.tb01351.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-012-0234-5"},{"key":"e_1_2_5_4_1","first-page":"721","article-title":"Pericardial effusion in the dog: a review of 42 cases","volume":"20","author":"Berg R. 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