{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T12:16:12Z","timestamp":1762604172906},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,4,26]],"date-time":"2019-04-26T00:00:00Z","timestamp":1556236800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Texas A&M University Ginn Foundation"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Small Animal Practice"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To compare the concentration of phosphatidylserine\u2010positive extracellular vesicles and phosphatidylserine\u2010derived procoagulant activity of canine haemoperitoneum fluids and packed red blood cell units.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Ten dogs with haemoperitoneum (neoplasia = 7; trauma = 1; other = 2) were recruited, and five non\u2010leukoreduced packed red blood cell units purchased. Supernatants were collected from haemoperitoneum samples and packed red blood cell units using a consistent centrifugation protocol. Phosphatidylserine\u2010positive extracellular vesicle concentrations were measured by flow cytometry and phosphatidylserine\u2010mediated procoagulant activity by a commercial thrombin generation assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Phosphatidylserine\u2010mediated procoagulant activity was significantly higher for supernatants collected from packed red blood cell units (median 54\u2009nM, range 53 to 60\u2009nM) than haemoperitoneum samples (median 43\u2009nM; range 7 to 51\u2009nM; P = 0.0007). By flow cytometry, the concentration of phosphatidylserine\u2010positive extracellular vesicles was not significantly different between packed red blood cell (median: 415\/\u03bcL, range 173 to 1331\/\u03bcL) and haemoperitoneum samples (median: 314\/\u03bcL, range 132 to 3880\/\u03bcL; P = 0.77).<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical Significance<\/jats:title><jats:p>This study does not suggest that shed abdominal blood contains more extracellular vesicles with phosphatidylserine\u2010mediated procoagulant activity than donor packed red blood cell units. Clinical studies to compare the effects of autologous transfusion of shed abdominal blood and packed red blood cell units on coagulation status and clinical outcome are required.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jsap.13002","type":"journal-article","created":{"date-parts":[[2019,4,26]],"date-time":"2019-04-26T09:04:30Z","timestamp":1556269470000},"page":"423-429","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Extracellular vesicle concentration and procoagulant activity of canine haemoperitoneum fluid and packed red blood cells"],"prefix":"10.1111","volume":"60","author":[{"given":"S.","family":"Sowy","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University  College Station Texas 77843 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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