{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:54:45Z","timestamp":1760550885368,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T00:00:00Z","timestamp":1563235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Small Animal Practice"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To evaluate matrix\u2010assisted laser desorption ionisation time of flight mass spectrometry (MALDI\u2010TOF MS) combined with the Sepsityper kit (Bruker Daltoniks GmbH, Bremen) for the direct detection of bacterial species from inoculated blood cultures from dogs and cats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Canine and feline blood samples were inoculated with typical sepsis\u2010causing bacteria such as <jats:italic>Staphylococcus intermedius, Staphylococcus aureus, Streptococcus canis, Enterococcus faecalis, Escherichia coli<\/jats:italic> and <jats:italic>Pseudomonas aeruginosa<\/jats:italic> at two distinct concentrations (each in triplicate), resulting in 72 blood culture bottles incubated at 37\u00b0C. Samples were comparatively analysed with MALDI\u2010TOF MS after preparation with the Sepsityper kit and also by standard bacteriology (culturing and biochemical characterisation).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Bacterial species identified from agar plates and by MALDI\u2010TOF MS from blood culture bottles were identical for all samples. The MALDI Biotyper software (Bruker Daltoniks) correctly identified all bacterial strains from inoculated canine and feline blood with analysis indicating very good precision.<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical Significance<\/jats:title><jats:p>MALDI\u2010TOF MS analysis combined with the Sepsityper kit is a reliable tool for a quick detection of veterinary\u2010relevant bacterial species directly from blood culture bottles. This approach could reduce the time for identification of critical species to only 24\u2009hours.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jsap.13056","type":"journal-article","created":{"date-parts":[[2019,7,17]],"date-time":"2019-07-17T05:34:50Z","timestamp":1563341690000},"page":"42-45","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Acceleration of the identification of sepsis\u2010inducing bacteria in cultures of\u00a0dog and cat blood"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4511-9537","authenticated-orcid":false,"given":"S.","family":"Ulrich","sequence":"first","affiliation":[{"name":"Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences LMU Munich  80539 Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Gottschalk","sequence":"additional","affiliation":[{"name":"Chair of Food Safety, Department of Veterinary Sciences LMU Munich  85764 Oberschleissheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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