{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:58:29Z","timestamp":1760551109748,"version":"3.40.5"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T00:00:00Z","timestamp":1586995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Small Animal Practice"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>First, to investigate the biological variability of N\u2010terminal pro\u2010B\u2010type natriuretic peptide (NT\u2010proBNP) in healthy Labrador retrievers and compare this with current laboratory recommendations for dilated cardiomyopathy screening. Second, to calculate a breed\u2010specific reference interval and validate it in a retrospective cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Plasma NT\u2010proBNP was measured in 51 clinically healthy Labrador retrievers at 0, 2, 4, 6 and 8\u2009weeks. Coefficient of variation for individual dogs over time, the coefficient of variation for the group at each time point and the index of individuality were calculated. A reference interval was derived and tested on a clinical dataset available from four UK cardiology referral centres.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Median NT\u2010proBNP was 865\u2009pmol\/L (315 to 2064\u2009pmol\/L). Mean individual coefficient of variation was 19% (95% CI: 16 to 21%) and group coefficient of variation was 43% (95% CI: 41 to 46%), with index of individuality at 0.44. The breed\u2010specific reference interval was 275 to 2100\u2009pmol\/L. In the validation group, 93% of NT\u2010proBNP measurements from healthy dogs were within the reference interval. NT\u2010proBNP measurements exceeded the reference interval in 82% of dogs with dilated cardiomyopathy. The upper bound of the reference interval (2100\u2009pmol\/L) had a positive predictive value of 90% and a negative predictive value of 87% for identification of dilated cardiomyopathy in this population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical Significance<\/jats:title><jats:p>Breed\u2010specific reference intervals might improve the diagnostic accuracy of NT\u2010proBNP measurement. Applying the currently recommended general cut\u2010off value to Labradors is likely to result in frequent false positives and diagnosis would be improved by application of the new breed\u2010specific reference interval calculated here.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jsap.13136","type":"journal-article","created":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T08:12:16Z","timestamp":1587024736000},"page":"368-373","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Long\u2010term biological variability and the generation of a new reference interval for plasma N\u2010terminal pro\u2010B\u2010type natriuretic peptide in Labrador retrievers"],"prefix":"10.1111","volume":"61","author":[{"given":"S.","family":"Gomart","sequence":"first","affiliation":[{"name":"Langford Vets University of Bristol Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Allaway","sequence":"additional","affiliation":[{"name":"MARS PetCare Ltd Waltham Centre for Pet Nutrition Waltham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Harrison","sequence":"additional","affiliation":[{"name":"MARS PetCare Ltd Waltham Centre for Pet Nutrition Waltham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Dickson","sequence":"additional","affiliation":[{"name":"HeartVets Porthcawl UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8215-1237","authenticated-orcid":false,"given":"J.","family":"Seo","sequence":"additional","affiliation":[{"name":"Royal Veterinary College London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Ferasin","sequence":"additional","affiliation":[{"name":"Lumbry Park Veterinary Specialists Alton UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6057-520X","authenticated-orcid":false,"given":"J. 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