{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T06:53:24Z","timestamp":1763621604637,"version":"3.40.5"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,1,7]],"date-time":"2024-01-07T00:00:00Z","timestamp":1704585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Small Animal Practice"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study aimed to assess the prevalence of blood\u2010borne infectious agents in healthy, client\u2010owned dogs from a blood donor population in Portugal and Spain, and to address the importance of a screening protocol on every donation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Client\u2010owned healthy dogs were tested before each donation on a veterinary blood bank. Blood samples from new potential donors, and from regular donors, were tested by real\u2010time polymerase chain reaction for <jats:italic>Leishmania<\/jats:italic> species, <jats:italic>Ehrlichia<\/jats:italic> species, <jats:italic>Brucella<\/jats:italic> species, <jats:italic>Babesia<\/jats:italic> species and <jats:italic>Anaplasma<\/jats:italic> species Serological tests were also performed for <jats:italic>Leishmania<\/jats:italic> species, <jats:italic>Ehrlichia<\/jats:italic> species and <jats:italic>Dirofilaria immitis<\/jats:italic>. All donors were tested for every infectious agent in each donation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The study found that out of a total of 8036 donors and 35,120 samples tested, 3.9% of blood donors tested positive for at least one of the agents, with the most prevalent being <jats:italic>Anaplasma<\/jats:italic> species (2.1%). Serological tests also revealed positive results in 14.0% of donors, with the highest percentage for <jats:italic>Leishmania<\/jats:italic> species (7.7%). Moreover, the study found that 28.2% of positive results were from dogs with negative results in donations performed 3 to 12\u2009months before, and 18.0% of positive results were recent infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Clinical Significance<\/jats:title><jats:p>These findings indicate a high prevalence of infectious agents in seemingly healthy, selected dogs eligible to become blood donors in the Iberian Peninsula, highlighting the importance of regular testing on every donation. This study emphasises the importance of a regular screening protocol for every donation instead of annual testing, as is commonly performed in veterinary medicine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jsap.13698","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T04:43:35Z","timestamp":1704689015000},"page":"176-180","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Prevalence of subclinical infectious agents in a blood donor population tested on every donation"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9527-1628","authenticated-orcid":false,"given":"B.","family":"Correia","sequence":"first","affiliation":[{"name":"Banco de Sangue Animal (NIV \u2013 N\u00facleo de Investiga\u00e7\u00e3o Veterin\u00e1ria), Rua Dr. Eduardo Santos Silva  n\u00b0261, AH 4200\u2010283 Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Magalh\u00e3es","sequence":"additional","affiliation":[{"name":"Banco de Sangue Animal (NIV \u2013 N\u00facleo de Investiga\u00e7\u00e3o Veterin\u00e1ria), Rua Dr. Eduardo Santos Silva  n\u00b0261, AH 4200\u2010283 Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Rocha","sequence":"additional","affiliation":[{"name":"Banco de Sangue Animal (NIV \u2013 N\u00facleo de Investiga\u00e7\u00e3o Veterin\u00e1ria), Rua Dr. Eduardo Santos Silva  n\u00b0261, AH 4200\u2010283 Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"BSA \u2013 Banco de Sangue Animal, Rua de Jo\u00e3o de Deus  n\u00b0741 4100\u2010462 Porto Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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